Botrel Bianca Mesquita Coelho, Abreu Daiane Cássia Pereira, Saczk Adelir Aparecida, Bazana Matheus Julien Ferreira, Coelho Sabrina Mesquita, E Rosa Priscila Vieira, Magriotis Zuy Maria, de Lima Rafael Machado Felix
Department of Chemistry, Federal University of Lavras, 37200-000 Lavras, MG, Brazil.
Department of Animal Science, Federal University of Lavras, 37200-000 Lavras, MG, Brazil.
Food Chem. 2017 Aug 15;229:674-679. doi: 10.1016/j.foodchem.2017.02.087. Epub 2017 Feb 21.
The SDME-GC/MS method was applied to residual determination of anesthetic menthol in fish. The extractions took place from the headspace of the sample using 1.8µL of octane as the extraction solvent. To obtain the ideal extraction condition, was used Response Surface Methodology, defining: extraction time 15min, temperature 30°C and salt 3g. The method showed LOD and LOQ of 0.021 and 1.56µgL respectively, recovery of 94% and R of 0.9997. The analyzes were performed on tilapia fillets anesthetized in five concentrations between 5 and 15×10µgL and with times of slaughter after anesthesia of 0, 12, 24 and 48h. It was determined that 48h is the required residual period for total metabolization of menthol in the fishes' organisms. This methodology becomes promising regarding the establishment of protocols to regulatory the use of menthol as an anesthetic in aquaculture.
采用顶空固相微萃取-气相色谱/质谱联用（SDME-GC/MS）法测定鱼类中麻醉剂薄荷醇的残留量。以1.8µL辛烷作为萃取溶剂，从样品顶空中进行萃取。为获得理想的萃取条件，采用响应面法，确定萃取时间为15分钟、温度为30°C、盐用量为3g。该方法的检测限（LOD）和定量限（LOQ）分别为0.021和1.56µg/L，回收率为94%，相关系数（R）为0.9997。对罗非鱼片进行分析，罗非鱼用5至15×10µg/L的五种浓度麻醉，麻醉后分别在0、12、24和48小时宰杀。结果确定48小时是薄荷醇在鱼体内完全代谢所需的残留期。该方法在制定水产养殖中使用薄荷醇作为麻醉剂的监管方案方面具有广阔前景。
