人脐带源基质：一种适用于组织工程应用的支架。
Human umbilical cord derived matrix: A scaffold suitable for tissue engineering application.
作者信息
Dan Pan, Velot Émilie, Mesure Benjamin, Groshenry Guillaume, Bacharouche Jalal, Decot Véronique, Menu Patrick
机构信息
UMR 7365, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), CNRS-Université de Lorraine, Vandœuvre-lès-Nancy, 54505, France.
UMR 7564, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, Université de Lorraine, Villers-lès-Nancy, 54600, France.
出版信息
Biomed Mater Eng. 2017;28(s1):S95-S100. doi: 10.3233/BME-171629.
BACKGROUND
Human tissue derived natural extracellular matrix (ECM) has great potential in tissue engineering.
OBJECTIVE
We sought to isolate extracellular matrix derived from human umbilical cord and test its potential in tissue engineering.
METHODS
An enzymatic method was applied to isolate and solubilized complete human umbilical cord derived matrix (hUCM). The obtained solution was analyzed for growth factors, collagen and residual DNA contents, then used to coat 2D and 3D surfaces for cell culture application.
RESULTS
The hUCM was successfully isolated with trypsin digestion to acquire a solution containing various growth factors and collagen but no residual DNA. This hUCM solution can form a coating on 2D and 3D substrates suitable cell culture.
CONCLUSION
We developed a new matrix derived from human source that can be further used in tissue engineering.
背景
源自人体组织的天然细胞外基质（ECM）在组织工程中具有巨大潜力。
目的
我们试图分离源自人脐带的细胞外基质，并测试其在组织工程中的潜力。
方法
采用酶法分离并溶解完整的人脐带衍生基质（hUCM）。对所得溶液进行生长因子、胶原蛋白和残留DNA含量分析，然后用于包被二维和三维表面以用于细胞培养。
结果
通过胰蛋白酶消化成功分离出hUCM，获得了一种含有多种生长因子和胶原蛋白但无残留DNA的溶液。这种hUCM溶液可在二维和三维基质上形成适合细胞培养的涂层。
结论
我们开发了一种源自人类的新型基质，可进一步用于组织工程。
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