Azadi L, Tavalaee M, Deemeh M R, Arbabian M, Nasr-Esfahani M H
Department of Reproductive Biotechnology. Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
Department of Reproductive Biotechnology. Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran; Isfahan Fertility and Infertility Center, Isfahan, Iran.
Cryo Letters. 2017 Jan/Feb;38(1):29-36.
BACKGROUND: Quercetin is a flavonoid with high reactive oxygen species (ROS) scavenging and ion chelating activity. It also enhances the activity of antioxidant enzymes and reduces enzymatic activity such as NADPH oxidase and NADH-dependent oxido-reductase. Tempol, as a superoxide dismutase mimetic agent, converts superoxide to less toxic hydrogen peroxide (HO), but cannot reduce highly toxic hydroxyl radicals in Fenton or Haber-Weiss reactions mediated with free iron or cupper.
The study was to compare the effect of Quercetin and Tempol in an optimized commercial cryo-protective media on ROS induced cryoinjury for the first time.
Following administration of these compounds during freezing process, sperm motility, viability, ROS production and DNA integrity were assessed before and after freezing/thawing process.
Data showed that 10 µM Quercetin and 5 µM Tempol significantly improved sperm motility and viability, but they together had no additive effect. Supplementation with Quercetin alone or combination of Quercetin with Tempol decrease the ROS concentration, but the reduction was not significant for Tempol alone compared to control group. Quercetin and Tempol significantly decrease DNA fragmentation.
The supplementation of Quercetin or Tempol, but not their combination improves the quality of cryopreserved human semen.
背景：槲皮素是一种具有高活性氧（ROS）清除和离子螯合活性的黄酮类化合物。它还能增强抗氧化酶的活性，并降低诸如NADPH氧化酶和NADH依赖性氧化还原酶等酶的活性。Tempol作为一种超氧化物歧化酶模拟剂，可将超氧化物转化为毒性较小的过氧化氢（HO），但在由游离铁或铜介导的芬顿或哈伯-维伊斯反应中，无法还原高毒性的羟基自由基。
本研究旨在首次比较槲皮素和Tempol在优化的商业冷冻保护介质中对ROS诱导的冷冻损伤的影响。
在冷冻过程中给予这些化合物后，评估冷冻/解冻前后精子的活力、存活率、ROS产生和DNA完整性。
数据显示，10μM槲皮素和5μM Tempol能显著提高精子活力和存活率，但它们共同作用时没有相加效应。单独补充槲皮素或槲皮素与Tempol联合使用可降低ROS浓度，但与对照组相比，单独使用Tempol时ROS浓度的降低不显著。槲皮素和Tempol显著降低DNA片段化。
补充槲皮素或Tempol，但不是它们的组合，可提高冷冻保存的人类精液质量。
