ST6GAL1在结直肠癌肿瘤进展中的差异表达。
Differential expression of ST6GAL1 in the tumor progression of colorectal cancer.
作者信息
Zhang Sen, Lu Jishun, Xu Zhijue, Zou Xia, Sun Xue, Xu Yingjiao, Shan Aidong, Lu Jiaoyang, Yan Xialin, Cui Yalu, Yan Wei, Du Yuguo, Gu Jianguo, Zheng Minhua, Feng Bo, Zhang Yan
机构信息
Department of General Surgery, Shanghai Minimally Invasive Surgery Center, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197, Ruijin Er Road, Shanghai 200025, China.
Key Laboratory of Systems Biomedicine (Ministry of Education), and Collaborative Innovation Center of Systems Biomedicine, Shanghai Center for Systems Biomedicine (SCSB), Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China; Division of Regulatory Glycobiology, Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, 4-4-1 Komatsushima, Aobaku, Sendai 981-8558, Miyagi, Japan.
出版信息
Biochem Biophys Res Commun. 2017 May 13;486(4):1090-1096. doi: 10.1016/j.bbrc.2017.03.167. Epub 2017 Apr 1.
Elevated expression of β-galactoside α2,6-sialyltranferase 1 (ST6GAL1) has been observed in colorectal cancer (CRC) and demonstrated to be important for its tumorigenesis. Here, we found that ST6GAL1 expression was significantly higher in non-metastatic tumors (stage I and II) than that in metastatic tumors (stage III and IV) using 62 pair-matched tumor/normal tissues. To elucidate the molecular mechanisms of how ST6GAL1 affected the CRC progression, we performed a global identification of the substrates of ST6GAL1 in the colon adenocarcinoma cell line SW480. A total of 318 membrane proteins were identified differentially affected by ST6GAL1 overexpression using metabolic labeling and proteomic analysis. Subsequent bioinformatic analysis revealed a list of potential substrates that might mediate the different functions of ST6GAL1 in CRC including cell movement, cell death and survival. Taken together, these results indicate a dynamic change in the expression of ST6GAL1 during the CRC progression and provide a list of sialylated proteins potentially relevant to the different functions of ST6GAL1 in CRC.
在结直肠癌（CRC）中已观察到β-半乳糖苷α2,6-唾液酸转移酶1（ST6GAL1）的表达升高，并且已证明其对肿瘤发生很重要。在此，我们使用62对匹配的肿瘤/正常组织发现，非转移性肿瘤（I期和II期）中ST6GAL1的表达明显高于转移性肿瘤（III期和IV期）。为了阐明ST6GAL1影响CRC进展的分子机制，我们在结肠腺癌细胞系SW480中对ST6GAL1的底物进行了全面鉴定。使用代谢标记和蛋白质组学分析，共鉴定出318种受ST6GAL1过表达差异影响的膜蛋白。随后的生物信息学分析揭示了一系列潜在底物，这些底物可能介导ST6GAL1在CRC中的不同功能，包括细胞运动、细胞死亡和存活。综上所述，这些结果表明ST6GAL1在CRC进展过程中的表达存在动态变化，并提供了一系列可能与ST6GAL1在CRC中的不同功能相关的唾液酸化蛋白清单。
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