Coronel Institute of Occupational Health, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105AZ, Amsterdam, the Netherlands.
Department of Dermatology-Allergology, VU University Medical Center, Amsterdam, the Netherlands.
Br J Dermatol. 2017 Jun;176(6):1533-1540. doi: 10.1111/bjd.15163. Epub 2017 Apr 6.
Recent studies have demonstrated allergen-specific differences in the gene expression of inflammatory mediators in patch tested skin.
To determine levels of various inflammatory mediators in the stratum corneum (SC) after patch testing with common contact allergens and the skin irritant sodium lauryl sulfate (SLS).
In total, 27 individuals who had previously patch tested positive to nickel, chromium, methylchloroisothiazolinone/methylisothiazolinone (MCI/MI) or para-phenylenediamine were retested and then patch tested with SLS and petrolatum, with petrolatum serving as the patch test control. At 72 h, the test sites were clinically graded and the SC samples collected on adhesive tape.
The levels of 18 of the 32 quantified mediators differed significantly from that of the control patches for at least one of the tested substances. SLS and MCI/MI induced the largest number of immunomediators. Interleukin (IL)-16 levels were significantly higher in patch test reactions in all allergens than they were in the controls, while no significant difference was detected for SLS. Furthermore, a strong negative correlation was found between strength of patch test reaction and IL-1α levels.
Cytokine profiles in the SC of patch tested skin did not show a distinct allergen-specific pattern. However, MCI/MI induced a larger and wider immune response than the other allergens, perhaps due to its potency as an irritant. The levels of IL-16 were significantly increased in patch test reactions to allergens but not to SLS; thus, they may help clinicians to differentiate between allergic contact dermatitis and irritant contact dermatitis.
最近的研究表明，在斑贴试验的皮肤中，炎症介质的基因表达存在过敏原特异性差异。
确定常见接触过敏原和皮肤刺激性物质十二烷基硫酸钠（SLS）斑贴试验后角质层（SC）中各种炎症介质的水平。
共 27 名先前对镍、铬、甲基氯异噻唑啉酮/甲基异噻唑啉酮（MCI/MI）或对苯二胺斑贴试验阳性的个体进行了重测，然后用 SLS 和凡士林进行斑贴试验，凡士林作为斑贴试验对照。72 小时后，对试验部位进行临床分级，并在粘性带上采集 SC 样本。
在至少一种测试物质中，32 种定量介质中有 18 种的水平与对照斑贴显著不同。SLS 和 MCI/MI 诱导的免疫介质数量最多。与对照组相比，所有过敏原的斑贴试验反应中白细胞介素（IL）-16 水平明显升高，而 SLS 则没有差异。此外，还发现斑贴试验反应强度与 IL-1α 水平之间存在强烈的负相关。
SC 中的细胞因子谱在斑贴试验皮肤中没有表现出明显的过敏原特异性模式。然而，MCI/MI 比其他过敏原引起更大和更广泛的免疫反应，这可能是由于其作为一种刺激性物质的效力。过敏原斑贴试验反应中 IL-16 水平显著升高，但 SLS 则没有；因此，它们可能有助于临床医生区分过敏性接触性皮炎和刺激性接触性皮炎。
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