Pinna Sara, Pasella Sara, Deiana Marta, Baralla Angela, Mannu Andrea, Masala Antonio Giovanni Emilio, Pileri Piera Veronica, Deiana Nicola, Scognamillo Fabrizio, Pala Carlo, Zinellu Angelo, Carru Ciriaco, Deiana Luca
Department of Biomedical Sciences, University of Sassari, Sassari, Italy.
Associazione "L'Isola dei Centenari", Sassari, Italy.
J Immunol Methods. 2017 Jul;446:37-46. doi: 10.1016/j.jim.2017.03.019. Epub 2017 Apr 5.
Systemic Lupus Erythematosus (SLE) is a systemic autoimmune disease with a broad spectrum of clinical presentations and incompletely understood pathogenesis. This autoimmune disease is characterized by alterations in both the innate and adaptive immune system that lead to the loss of immunologic tolerance. In autoimmune diseases particularly in SLE, early diagnosis, flare or remission phases can be difficult to identify. Proteomics can help to find new therapeutic targets and it also could help to better understand the cellular mechanisms. The aim of this study was to observe the variations in plasma and Peripheral Blood Mononuclear Cells (PBMCs) proteome in order to increase our knowledge about pathogenesis and to find possible diagnostic markers and/or therapeutic targets for improving diagnosis and treatment. The comparative proteomic analyses showed that several proteins were differentially expressed in the PBMCs from SLE patients. Among these, PRDX2 may be used as candidate biomarker or target protein for further investigations. In plasma, we showed that plasma clusterin levels increased in SLE patients compared to healthy controls, but this increase is not statistically significant. These proteomic results provide suggestions for understanding the molecular mechanisms of SLE, as well as the physiological changes correlated with SLE disease.
系统性红斑狼疮（SLE）是一种全身性自身免疫性疾病，临床表现多样，发病机制尚未完全明确。这种自身免疫性疾病的特征是先天性和适应性免疫系统均发生改变，导致免疫耐受丧失。在自身免疫性疾病中，尤其是SLE，早期诊断、病情发作或缓解阶段可能难以识别。蛋白质组学有助于寻找新的治疗靶点，也有助于更好地理解细胞机制。本研究的目的是观察血浆和外周血单个核细胞（PBMC）蛋白质组的变化，以增加我们对发病机制的了解，并寻找可能的诊断标志物和/或治疗靶点，以改善诊断和治疗。比较蛋白质组学分析表明，SLE患者的PBMC中有几种蛋白质表达存在差异。其中，PRDX2可作为进一步研究的候选生物标志物或靶蛋白。在血浆中，我们发现与健康对照相比，SLE患者血浆中的簇集素水平升高，但这种升高无统计学意义。这些蛋白质组学结果为理解SLE的分子机制以及与SLE疾病相关的生理变化提供了线索。
