寡聚吡咯甲酰胺下调家蚕 Broad-Complex 基因表达以提高丝产量。
Knockdown of Broad-Complex Gene Expression of Bombyx mori by Oligopyrrole Carboxamides Enhances Silk Production.
机构信息
Department of Organic Chemistry, Indian Institute of Science, Bangalore, 560 012, India.
Department of Biotechnology, GITAM Institute of Science, GITAM University, Visakhapatnam, 530 045, India.
出版信息
Sci Rep. 2017 Apr 11;7(1):805. doi: 10.1038/s41598-017-00653-3.
Bombyx mori (B. mori) is important due to its major role in the silk production. Though DNA binding ligands often influence gene expression, no attempt has been made to exploit their use in sericulture. The telomeric heterochromatin of B. mori is enriched with 5'-TTAGG-3' sequences. These sequences were also found to be present in several genes in the euchromatic regions. We examined three synthetic oligopyrrole carboxamides that target 5'-TTAGG-3' sequences in controlling the gene expression in B. mori. The ligands did not show any defect or feeding difference in the larval stage, crucial for silk production. The ligands caused silencing of various isoforms of the broad-complex transcription factor and cuticle proteins which resulted in late pupal developmental defects. Furthermore, treatment with such drugs resulted in statistically enhanced cocoon weight, shell weight, and silk yield. This study shows for the first time use of oligopyrrole carboxamide drugs in controlling gene expression in B. mori and their long term use in enhancing silk production.
家蚕（B. mori）在丝绸生产中起着重要作用。尽管 DNA 结合配体通常会影响基因表达，但尚未尝试利用它们在家蚕养殖中的应用。家蚕的端粒异染色质富含 5'-TTAGG-3' 序列。这些序列也被发现存在于常染色质区域的几个基因中。我们研究了三种针对 5'-TTAGG-3' 序列的合成寡吡咯酰胺，以控制家蚕的基因表达。这些配体在幼虫期（对丝绸生产至关重要）没有表现出任何缺陷或摄食差异。配体导致广谱复合物转录因子和表皮蛋白的各种同工型沉默，导致后期蛹发育缺陷。此外，用这种药物处理会导致茧重、壳重和丝产量在统计学上显著增加。这项研究首次表明，寡吡咯酰胺药物可用于控制家蚕的基因表达，并可长期用于提高丝绸产量。
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