Zhou Qiang, Dong Yongbin, Shi Qingling, Zhang Long, Chen Huanqing, Hu Chunhui, Li Yuling
College of Agronomy, Henan Agricultural University, Collaborative Innovation Center of Henan Grain Crops, National Key Laboratory of Wheat and Maize Crop Science, 95 Wenhua Rd, Zhengzhou, 450002, China.
Mol Genet Genomics. 2017 Aug;292(4):871-881. doi: 10.1007/s00438-017-1318-0. Epub 2017 Apr 12.
Grain weight, one of the important factors to determine corn yield, is a typical quantitative inheritance trait. However, the molecular genetic basis of grain weight still remains limited. In our previous researches, a major QTL associated with grain weight, qGW1.05, has been identified between SSR markers umc1601 and umc1754 at bin locus 1.05-1.06 in maize. Here, its genetic and environmental stabiliteis were verified using a BCF population to identify the effect of qGW1.05 on grain weight. Further, qGW1.05-NILs were obtained by MAS successfully. Via a large BCF segregation population, together with polymorphic microsatellite markers developed between the parents to screen the genotype of the recombinant plants, qGW1.05 was positioned to a 1.11 Mb genome interval. Furthermore, the progenies of 15 recombinants were tested to confirm the effect of qGW1.05 on grain weight. Combining collinearity among cereal crops and genome annotation, the several candidate genes taking part in grain development were identified in the qGW1.05 region. In this study, qGW1.05 was limited to a 1.11 Mb region on chromosome 1, which established the foundation for understanding the molecular basis underlying kernel development and improving grain weight through MAS using the tightly flanking molecular markers in maize.
粒重是决定玉米产量的重要因素之一，是典型的数量遗传性状。然而，粒重的分子遗传基础仍然有限。在我们之前的研究中，已在玉米第1.05 - 1.06染色体bin位点的SSR标记umc1601和umc1754之间鉴定出一个与粒重相关的主效QTL，即qGW1.05。在此，利用一个BCF群体验证了其遗传稳定性和环境稳定性，以确定qGW1.05对粒重的影响。此外，通过MAS成功获得了qGW1.05近等基因系。通过一个大的BCF分离群体，结合亲本间开发的多态性微卫星标记筛选重组植株的基因型，将qGW1.05定位到一个1.11 Mb的基因组区间。此外，对15个重组体的后代进行了测试，以确认qGW1.05对粒重的影响。结合谷类作物间的共线性和基因组注释，在qGW1.05区域鉴定出几个参与籽粒发育的候选基因。在本研究中，qGW1.05被定位到第1染色体上一个1.11 Mb的区域，这为理解玉米籽粒发育的分子基础以及利用紧密连锁的分子标记通过MAS提高粒重奠定了基础。
