Coexistence of melanin-concentrating hormone and alpha-melanocyte-stimulating hormone immunoreactivities in the central nervous system of the locust, Locusta migratoria.

The distribution of melanin concentrating hormone (MCH) in the central nervous system of the locust Locusta migratoria was studied by the indirect immunofluorescence technique, using antibodies against salmon MCH. Most MCH-immunoreactive perikarya were found in the optic lobes at both sides of the brain, dorsally with respect to the lamina ganglionaris. The same neurons also contain alpha-melanocyte-stimulating hormone (alpha-MSH)-like material. In addition, a moderate number of MCH-like neurons, which were devoid of alpha-MSH-immunoreactive substances, was observed in the pars intercerebralis. Bright immunofluorescent fibers were visualized in various regions of the central nervous system of the locust: the optic lobes, the ocelli, the proto-and deuterocerebrum, the subesophageal connectives and the corpora cardiaca. In the ventral nerve cord and the subesophageal ganglion, where alpha-MSH-like cell bodies were encountered, MCH immunoreactive perikarya were absent and immunoreactive fibers were scarce. The coexistence of MCH and alpha-MSH-immunoreactive material within the same specific neurons might indicate an evolutionary relationship of both peptides.