Isachenko Vladimir, Sanchez Raul, Mallmann Peter, Rahimi Gohar, Isachenko Evgenia
Department of Obsterics and Gynecology, Medical Faculty, University Maternal Hospital, Cologne University, Kerpener Str 34, 50931, Cologne, Germany.
Department of Preclinical Science, Facultad de Medicina, Center for Traslation Medicine, Universidad de la Frontera, Avenida Alemania, 0458, Temuco, Chile.
Methods Mol Biol. 2017;1568:79-84. doi: 10.1007/978-1-4939-6828-2_6.
The aim of this chapter was to describe the standardized aseptic technology of permeable cryoprotectant-free vitrification of human spermatozoa in capillaries (for ICSI or IVF in microvolume). Spermatozoa, vitrified by this technology, are free from seminal plasma owing to swim-up procedure preceding vitrification and are free from permeable cryoprotectants. They are ready for further use immediately after warming without any additional treatment.
本章的目的是描述毛细管中人类精子无渗透冷冻保护剂玻璃化的标准化无菌技术（用于微体积的卵胞浆内单精子注射或体外受精）。通过该技术玻璃化的精子，由于在玻璃化之前进行了上游处理，不含精浆且不含渗透冷冻保护剂。解冻后无需任何额外处理即可立即进一步使用。
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