Iwamatsu T, Yoshimoto Y, Hiramoto Y
Department of Biology, Aichi University of Education, Kariya, Japan.
Dev Biol. 1988 Sep;129(1):191-7. doi: 10.1016/0012-1606(88)90173-x.
The reaction time of Ca2+ release from cytoplasmic stores induced by microinjection of inositol 1,4,5-trisphosphate (IP3), calcium ionophore A23187, Ca2+, Sr2+, Ba2+, and cyclic guanosine 5'-monophosphate (cGMP) in Oryzias latipes eggs in Ca2+-free medium was measured by the luminescence of aequorin injected into the egg. Microinjection of IP3 or calcium ionophore induced rapid Ca2+ release without a time lag, while microinjection of either Ca2+ or cGMP required a time lag of 5-30 sec for Ca2+ release. Following microinjection of both IP3 and Ca2+, Ca2+ release commenced in a cytoplasmic region close to the egg surface. These results suggest that in the medaka egg, cytoplasmic Ca2+ induces Ca2+ release from cytoplasmic stores indirectly, probably via a membrane factor such as IP3.
在无钙培养基中，通过向青鳉鱼卵中注射水母发光蛋白的发光来测量由显微注射肌醇1,4,5 -三磷酸（IP3）、钙离子载体A23187、Ca2 +、Sr2 +、Ba2 +和环磷酸鸟苷（cGMP）诱导的细胞质储存中Ca2 +释放的反应时间。显微注射IP3或钙离子载体可诱导Ca2 +迅速释放且无时间延迟，而显微注射Ca2 +或cGMP时，Ca2 +释放需要5 - 30秒的时间延迟。在显微注射IP3和Ca2 +后，Ca2 +释放在靠近卵表面的细胞质区域开始。这些结果表明，在青鳉鱼卵中，细胞质Ca2 +可能通过诸如IP3等膜因子间接诱导细胞质储存中Ca2 +的释放。
