Lee Jae Won, An Hyoseok, Lee Kuen Yong
Department of Bioengineering, Hanyang University, Seoul 04763, Republic of Korea.
Department of Bioengineering, Hanyang University, Seoul 04763, Republic of Korea; Institute of Nano Science and Technology, Hanyang University, Seoul 04763, Republic of Korea.
Colloids Surf B Biointerfaces. 2017 Jul 1;155:229-237. doi: 10.1016/j.colsurfb.2017.04.014. Epub 2017 Apr 9.
Control of stem cell fate and phenotype using biomimetic synthetic extracellular matrices (ECMs) is an important tissue engineering approach. Many studies have focused on improving cell-matrix interactions. However, proper control of cell-cell interactions using synthetic ECMs could be critical for tissue engineering, especially with undifferentiated stem cells. In this study, alginate hydrogels were modified with a peptide derived from the low-density lipoprotein receptor-related protein 5 (LRP5), which is known to bind to N-cadherin, as a cell-cell interaction motif. In vitro changes in the morphology and differentiation of mouse bone marrow stromal cells (D1 stem cells) cultured in LRP5-alginate hydrogels were investigated. LRP5-alginate gels successfully induced stem cell aggregation and enhanced chondrogenic differentiation of D1 stem cells, compared to RGD-alginate gels, at low cell density. This approach to tailoring synthetic biomimetic ECMs using cell-cell interaction motifs may be critical in tissue engineering approaches using stem cells.
利用仿生合成细胞外基质（ECM）控制干细胞命运和表型是一种重要的组织工程方法。许多研究都集中在改善细胞与基质的相互作用上。然而，使用合成ECM对细胞间相互作用进行适当控制对于组织工程可能至关重要，尤其是对于未分化的干细胞。在本研究中，藻酸盐水凝胶用一种源自低密度脂蛋白受体相关蛋白5（LRP5）的肽进行修饰，已知该肽可与N-钙黏蛋白结合，作为细胞间相互作用基序。研究了在LRP5-藻酸盐水凝胶中培养的小鼠骨髓基质细胞（D1干细胞）的形态和分化的体外变化。与RGD-藻酸盐水凝胶相比，LRP5-藻酸盐水凝胶在低细胞密度下成功诱导了干细胞聚集并增强了D1干细胞的软骨生成分化。这种利用细胞间相互作用基序定制合成仿生ECM的方法在使用干细胞的组织工程方法中可能至关重要。
