Hara Y, Urayama O, Kawakami K, Nojima H, Nagamune H, Kojima T, Ohta T, Nagano K, Nakao M
Dept of Biochemistry, Tokyo Medical and Dental University, Japan.
Prog Clin Biol Res. 1988;268A:73-8.
The cDNA encoding the third type of alpha-subunit of NaK-ATPase was found in rat brain cDNA library. The deduced amino acid sequence was very similar to those of alpha- and alpha plus-subunits. The third one was suggested to be highly ouabain-sensitive from its aminoacid sequence. From Northern blot analysis the third type of the subunit was not expressed in adult rat kidney and heart.
在大鼠脑cDNA文库中发现了编码钠钾-ATP酶第三种α亚基类型的cDNA。推导的氨基酸序列与α亚基和α加亚基的序列非常相似。从其氨基酸序列推测第三种类型对哇巴因高度敏感。通过Northern印迹分析,该亚基的第三种类型在成年大鼠肾脏和心脏中不表达。