一种标准化的体外猪模型的特征描述，用于评估在使用不同硅油和平衡盐溶液填塞物进行玻璃体切割术后的短期眼压变化和小梁网活力。

Characterization of a Standardized Ex-vivo Porcine Model to Assess Short Term Intraocular Pressure Changes and Trabecular Meshwork Vitality After Pars Plana Vitrectomy with Different Silicone Oil and BSS Tamponades.

作者信息

Ebner Martina, Mariacher Siegfried, Hurst José, Szurman Peter, Schnichels Sven, Spitzer Martin S, Januschowski Kai

机构信息

a Knappschaft Eye Clinic Sulzbach, Knappschaft Hospital Saar , Sulzbach , Germany.

b University Eye Hospital Tuebingen, Center for Ophthalmology , Tuebingen , Germany.

出版信息

Curr Eye Res. 2017 Aug;42(8):1130-1135. doi: 10.1080/02713683.2017.1297461. Epub 2017 Apr 25.

DOI:10.1080/02713683.2017.1297461

PMID:28441060

Abstract

PURPOSE

The aim of this study was to characterize a standardized porcine ex-vivo testing system for intraocular pressure (IOP) monitoring after vitrectomy with different endotamponades.

METHODS

Twenty-four pig eyes, six per endotamponade group were obtained immediately postmortem. After pars plana vitrectomy, vitreous substitutes (silicone oil 1000 mPas, 2000 mPas, 5000 mPas, and Balanced Salt Solution (BSS)) were instillated and IOP was observed over 24-hours. Infusion pumps with Dulbecco's Modified Eagle Medium (DMEM) simulated a constant aqueous humor circulation. A histological examination of the trabecular meshwork with DAPI- and TUNEL-staining was performed to detect the amount of apoptotic cells.

RESULTS

TUNEL-assay showed a mean cell death rate of 3.78% (SD ± 1.46%) for silicone oil endotamponades compared to 5.05% (SD ± 2.18%) in BSS group. One-way ANOVA (p = 0.425) showed no significant difference between both groups. Mean IOP in silicone oil endotamponades was 9.50 mmHg (SD ± 1.68 mmHg) at baseline, 13.23 mmHg (SD ± 0.79 mmHg) after 1 hour, 18.46 mmHg (SD ± 2.13 mmHg) after 12 hours and 15.51 mmHg (SD ± 2.82 mmHg) 24 hours after instillation. A comparison of all silicone oil groups (one-way ANOVA, Bonferroni post-hoc test, p = 0.269 to 1.000) didn't reveal significant differences in mean IOP.

CONCLUSION

The standardized ex-vivo porcine model represents an effective alternative to the in-vivo testing in animals. Maintaining the trabecular and uveoscleral outflow pathway enables a pseudo in-vivo analysis.

摘要

目的

本研究的目的是对一种标准化的猪离体测试系统进行特性描述，该系统用于在玻璃体切除术后使用不同的眼内填充物监测眼压（IOP）。

方法

在猪死后立即获取24只猪眼，每个眼内填充物组6只。在进行扁平部玻璃体切除术后，滴注玻璃体替代物（1000 mPas、2000 mPas、5000 mPas的硅油以及平衡盐溶液（BSS）），并在24小时内观察眼压。使用含 Dulbecco改良 Eagle培养基（DMEM）的输液泵模拟恒定的房水循环。对小梁网进行DAPI和TUNEL染色的组织学检查，以检测凋亡细胞的数量。

结果

TUNEL检测显示，硅油眼内填充物组的平均细胞死亡率为3.78%（标准差±1.46%），而BSS组为5.05%（标准差±2.18%）。单因素方差分析（p = 0.425）显示两组之间无显著差异。硅油眼内填充物组的平均眼压在基线时为9.50 mmHg（标准差±1.68 mmHg），滴注后1小时为13.23 mmHg（标准差±0.79 mmHg），12小时后为18.46 mmHg（标准差±2.13 mmHg），24小时后为15.51 mmHg（标准差±2.82 mmHg）。对所有硅油组进行比较（单因素方差分析，Bonferroni事后检验，p = 0.269至1.000）未发现平均眼压有显著差异。