Fallath Thorya, Kidd Brendan N, Stiller Jiri, Davoine Celine, Björklund Stefan, Manners John M, Kazan Kemal, Schenk Peer M
Plant-Microbe Interactions Laboratory, School of Agriculture and Food Sciences, The University of Queensland, St Lucia, Australia.
CSIRO Agriculture and Food, Queensland Bioscience Precinct, St Lucia, Australia.
PLoS One. 2017 Apr 25;12(4):e0176022. doi: 10.1371/journal.pone.0176022. eCollection 2017.
The conserved protein complex known as Mediator conveys transcriptional signals by acting as an intermediary between transcription factors and RNA polymerase II. As a result, Mediator subunits play multiple roles in regulating developmental as well as abiotic and biotic stress pathways. In this report we identify the head domain subunits MEDIATOR18 and MEDIATOR20 as important susceptibility factors for Fusarium oxysporum infection in Arabidopsis thaliana. Mutants of MED18 and MED20 display down-regulation of genes associated with jasmonate signaling and biosynthesis while up-regulation of salicylic acid associated pathogenesis related genes and reactive oxygen producing and scavenging genes. We propose that MED18 and MED20 form a sub-domain within Mediator that controls the balance of salicylic acid and jasmonate associated defense pathways.
被称为中介体的保守蛋白复合体通过充当转录因子与RNA聚合酶II之间的中间体来传递转录信号。因此，中介体亚基在调节发育以及非生物和生物胁迫途径中发挥多种作用。在本报告中，我们确定头部结构域亚基MEDIATOR18和MEDIATOR20是拟南芥对尖孢镰刀菌感染的重要易感因子。MED18和MED20的突变体显示与茉莉酸信号传导和生物合成相关的基因下调，而水杨酸相关的病程相关基因以及活性氧产生和清除基因上调。我们提出，MED18和MED20在中介体内形成一个子结构域，控制水杨酸和茉莉酸相关防御途径的平衡。
