大鼠心脏中的5'-核苷酸酶。存在两种具有不同底物特异性的可溶性酶的证据。
5'-Nucleotidases in rat heart. Evidence for the occurrence of two soluble enzymes with different substrate specificities.
作者信息
Truong V L, Collinson A R, Lowenstein J M
机构信息
Graduate Department of Biochemistry, Brandeis University, Waltham, MA 02254.
出版信息
Biochem J. 1988 Jul 1;253(1):117-21. doi: 10.1042/bj2530117.
Abstract
Chromatography of soluble proteins from rat heart on phosphocellulose columns separates two 5'-nucleotidases. The first to emerge from the column shows a preference for AMP over IMP as substrate, whereas the second shows a preference for IMP over AMP. The properties of the IMP-preferring enzyme, including the conditions under which it is eluted from phosphocellulose columns, show it to be the enzyme studied by Itoh, Oka & Ozasa [Biochem. J. (1986) 235, 847-851]. The kinetic properties of the AMP-preferring enzyme indicate that it is likely to be the enzyme responsible for the production of adenosine under conditions of hypoxia and increased work load, and with metabolic stresses such as a high load of acetate.
摘要
用磷酸纤维素柱对大鼠心脏的可溶性蛋白质进行色谱分析，可分离出两种5'-核苷酸酶。首先从柱中洗脱出来的酶对AMP作为底物的偏好高于IMP，而第二种酶对IMP作为底物的偏好高于AMP。偏好IMP的酶的特性，包括其从磷酸纤维素柱上洗脱的条件，表明它就是Itoh、Oka和Ozasa [《生物化学杂志》(1986年)235卷，847 - 851页]所研究的酶。偏好AMP的酶的动力学特性表明，在缺氧、工作负荷增加以及存在代谢应激（如高负荷乙酸盐）的情况下，它可能是负责生成腺苷的酶。
相似文献
1
5'-Nucleotidases in rat heart. Evidence for the occurrence of two soluble enzymes with different substrate specificities.大鼠心脏中的5'-核苷酸酶。存在两种具有不同底物特异性的可溶性酶的证据。
Biochem J. 1988 Jul 1;253(1):117-21. doi: 10.1042/bj2530117.
2
AMP and IMP dephosphorylation by soluble high- and low-Km 5'-nucleotidases.可溶性高亲和力和低亲和力5'-核苷酸酶对AMP和IMP的去磷酸化作用。
Am J Physiol. 1989 Mar;256(3 Pt 1):E386-91. doi: 10.1152/ajpendo.1989.256.3.E386.
3
The pigeon heart 5'-nucleotidase responsible for ischaemia-induced adenosine formation.负责缺血诱导腺苷形成的鸽心脏5'-核苷酸酶。
Biochem J. 1988 Jul 1;253(1):123-30. doi: 10.1042/bj2530123.
4
Diadenosine tetraphosphate activates cytosol 5'-nucleotidase.四磷酸二腺苷激活胞质5'-核苷酸酶。
Biochem Biophys Res Commun. 1986 Jul 16;138(1):261-7. doi: 10.1016/0006-291x(86)90274-3.
5
Partial purification and properties of an AMP-specific soluble 5'-nucleotidase from pigeon heart.鸽心AMP特异性可溶性5'-核苷酸酶的部分纯化及性质
Biochem J. 1990 May 15;268(1):117-22. doi: 10.1042/bj2680117.
6
Regulation of soluble 5'-nucleotidase I from rabbit heart.
Adv Exp Med Biol. 1989;253B:107-11. doi: 10.1007/978-1-4684-5676-9_17.
7
Distinct roles for recombinant cytosolic 5'-nucleotidase-I and -II in AMP and IMP catabolism in COS-7 and H9c2 rat myoblast cell lines.重组胞质5'-核苷酸酶I和II在COS-7和H9c2大鼠成肌细胞系中AMP和IMP分解代谢中的不同作用。
J Biol Chem. 2000 Apr 21;275(16):11666-71. doi: 10.1074/jbc.275.16.11666.
8
IMP degradative capacity in rat skeletal muscle fiber types.大鼠骨骼肌纤维类型中的肌苷酸降解能力。
Mol Cell Biochem. 1999 Sep;199(1-2):111-7. doi: 10.1023/a:1006924318114.
9
Soluble forms of 5'-nucleotidase in rat and human heart.
Am J Physiol. 1996 Apr;270(4 Pt 2):H1493-500. doi: 10.1152/ajpheart.1996.270.4.H1493.
10
Regulation of cytosol 5'-nucleotidase by adenylate energy charge.腺苷酸能荷对胞质5'-核苷酸酶的调节
Biochim Biophys Acta. 1981 May 14;659(1):31-7. doi: 10.1016/0005-2744(81)90268-0.
引用本文的文献
1
Structural Basis of the Molecular Switch between Phosphatase and Mutase Functions of Human Phosphomannomutase 1 under Ischemic Conditions.缺血条件下人磷酸甘露糖变位酶1磷酸酶与变位酶功能之间分子开关的结构基础
Biochemistry. 2018 Jun 26;57(25):3480-3492. doi: 10.1021/acs.biochem.8b00223. Epub 2018 May 11.
2
Oxidative inactivation of brain ecto-5'-nucleotidase by thiols/Fe2+ system.硫醇/Fe2+ 体系对脑外5'-核苷酸酶的氧化失活作用
Neurochem Res. 2000 Nov;25(11):1475-84. doi: 10.1023/a:1007624125136.
3
Evidence for control of adenosine metabolism in rat oxidative skeletal muscle by changes in pH.大鼠氧化型骨骼肌中pH值变化对腺苷代谢调控的证据。
J Physiol. 2000 Feb 1;522 Pt 3(Pt 3):467-77. doi: 10.1111/j.1469-7793.2000.t01-1-00467.x.
4
The effect of muscle contraction on the regulation of adenosine formation in rat skeletal muscle cells.肌肉收缩对大鼠骨骼肌细胞中腺苷形成调节的影响。
J Physiol. 1999 Aug 1;518 ( Pt 3)(Pt 3):761-8. doi: 10.1111/j.1469-7793.1999.0761p.x.
5
Stimulation of alpha 1-adrenoceptors and protein kinase C-mediated activation of ecto-5'-nucleotidase in rat hearts in vivo.体内大鼠心脏中α1-肾上腺素能受体的刺激及蛋白激酶C介导的ecto-5'-核苷酸酶激活
J Physiol. 1997 Aug 15;503 ( Pt 1)(Pt 1):119-27. doi: 10.1111/j.1469-7793.1997.119bi.x.
6
Structure-activity relationship of cytoplasmic 5'-nucleotidase substrate sites.细胞质5'-核苷酸酶底物位点的构效关系。
Biochem J. 1996 Mar 15;314 ( Pt 3)(Pt 3):1001-7. doi: 10.1042/bj3141001.
7
Effect of 5'-deoxy-5'-isobutylthioadenosine on formation and release of adenosine from neonatal and adult rat ventricular myocytes.5'-脱氧-5'-异丁硫基腺苷对新生及成年大鼠心室肌细胞中腺苷生成与释放的影响。
Biochem J. 1993 May 1;291 ( Pt 3)(Pt 3):833-9. doi: 10.1042/bj2910833.
8
Mechanisms of elevation of adenosine levels in anoxic hepatocytes.缺氧肝细胞中腺苷水平升高的机制。
Biochem J. 1993 Mar 15;290 ( Pt 3)(Pt 3):671-7. doi: 10.1042/bj2900671.
9
Reconstitution of 5'-nucleotidase of bull seminal plasma in spin-labeled liposomes.
J Membr Biol. 1994 Oct;142(1):137-44. doi: 10.1007/BF00233391.
10
28 kDa adenosine-binding proteins of brain and other tissues.大脑及其他组织的28千道尔顿腺苷结合蛋白。
Biochem J. 1989 Mar 15;258(3):653-61. doi: 10.1042/bj2580653.
本文引用的文献
1
2
Prolonged myocardial nucleotide depletion after brief ischemia in the open-chest dog.开胸犬短暂缺血后心肌核苷酸的长期耗竭
Am J Physiol. 1982 May;242(5):H818-26. doi: 10.1152/ajpheart.1982.242.5.H818.
3
Adenine nucleotide metabolism and compartmentalization in isolated adult rat heart cells.成年大鼠离体心脏细胞中的腺嘌呤核苷酸代谢与区室化
Circ Res. 1984 May;54(5):536-46. doi: 10.1161/01.res.54.5.536.
4
Studies on some molecular properties of cytosol 5'-nucleotidase from rat liver.大鼠肝脏胞质5'-核苷酸酶某些分子特性的研究。
Biochim Biophys Acta. 1982 May 5;716(1):110-3. doi: 10.1016/0304-4165(82)90208-2.
5
Different sites of adenosine formation in the heart.心脏中腺苷形成的不同位点。
Am J Physiol. 1981 Jun;240(6):H963-70. doi: 10.1152/ajpheart.1981.240.6.H963.
6
Purification and some properties of cytosol 5'-nucleotidase from rat liver.大鼠肝脏胞质溶胶5'-核苷酸酶的纯化及某些性质
Biochim Biophys Acta. 1981 Feb 13;657(2):402-10. doi: 10.1016/0005-2744(81)90326-0.
7
Adenylate deaminase. 3. Regulation of deamination pathways in extracts of rat heart and lung.腺苷酸脱氨酶。3. 大鼠心脏和肺提取物中脱氨途径的调节。
J Biol Chem. 1967 Nov 25;242(22):5281-8.
8
Properties of 5'-nucleotidase from avian heart.禽心脏5'-核苷酸酶的特性
Biochemistry. 1972 Jan 18;11(2):223-9. doi: 10.1021/bi00752a013.
9
Measurement of inorganic orthophosphate in biological materials: extraction properties of butyl acetate.生物材料中无机正磷酸盐的测定：乙酸丁酯的萃取特性
Anal Biochem. 1974 Aug;60(2):489-504. doi: 10.1016/0003-2697(74)90259-0.
10
Regulation of rat heart cytosol 5'-nucleotidase by adenylate energy charge.腺苷酸能荷对大鼠心脏胞质5'-核苷酸酶的调节作用。
Biochem J. 1986 May 1;235(3):847-51. doi: 10.1042/bj2350847.
Zotero 插件