Rutigliano Heloisa M, Wilhelm Amanda, Hall Justin, Shi Bi, Meng Qinggang, Stott Rusty, Bunch Thomas D, White Kenneth L, Davies Christopher J, Polejaeva Irina A
Department of Animal, Dairy and Veterinary Sciences, Utah State University, 4815 Old Main Hill, Logan, UT 84322, USA.
Reprod Fertil Dev. 2017 Apr;29(4):646-657. doi: 10.1071/RD15103.
The present retrospective study investigated pregnancy rates, the incidence of pregnancy loss and large offspring syndrome (LOS) and immune-related gene expression of sheep and goat somatic cell nuclear transfer (SCNT) pregnancies. We hypothesised that significantly higher pregnancy losses observed in sheep compared with goat SCNT pregnancies are due to the increased amounts of T-helper 1 cytokines and proinflammatory mediators at the maternal-fetal interface. Sheep and goat SCNT pregnancies were generated using the same procedure. Control pregnancies were established by natural breeding. Although SCNT pregnancy rates at 45 days were similar in both species, pregnancy losses between 45 and 60 days of gestation and the incidence of LOS were significantly greater in sheep than in goats. At term, the expression of proinflammatory genes in sheep SCNT placentas was increased, whereas that in goats was similar to that in control animals. Genes with altered expression in sheep SCNT placentas included cytotoxic T-lymphocyte-associated protein 4 (CTLA4), interleukin 2 receptor alpha (IL2RA), cluster of differentiation 28 (CD28), interferon gamma (IFNG), interleukin 6 (IL6), interleukin 10 (IL10), transforming growth factor beta 1 (TGFB1), tumor necrosis factor alpha (TNF-α), interleukin 1 alpha (IL1A) and chemokine (C-X-C motif) ligand 8 (CXCL8). Major histocompatibility complex-I protein expression was greater in sheep and goat SCNT placentas at term than in control pregnancies. An unfavourable immune environment is present at the maternal-fetal interface in sheep SCNT pregnancies.
本回顾性研究调查了绵羊和山羊体细胞核移植（SCNT）妊娠的妊娠率、妊娠丢失和巨大后代综合征（LOS）的发生率以及免疫相关基因表达。我们假设，与山羊SCNT妊娠相比，绵羊中观察到的显著更高的妊娠丢失是由于母胎界面处T辅助1细胞因子和促炎介质的量增加。绵羊和山羊SCNT妊娠采用相同程序产生。通过自然配种建立对照妊娠。尽管两种物种在45天时的SCNT妊娠率相似，但绵羊在妊娠45至60天期间的妊娠丢失和LOS发生率显著高于山羊。足月时，绵羊SCNT胎盘促炎基因的表达增加，而山羊的表达与对照动物相似。绵羊SCNT胎盘表达改变的基因包括细胞毒性T淋巴细胞相关蛋白4（CTLA4）、白细胞介素2受体α（IL2RA）、分化簇28（CD28）、干扰素γ（IFNG）、白细胞介素6（IL6）、白细胞介素10（IL10）、转化生长因子β1（TGFB1）、肿瘤坏死因子α（TNF-α）、白细胞介素1α（IL1A）和趋化因子（C-X-C基序）配体8（CXCL8）。足月时，绵羊和山羊SCNT胎盘的主要组织相容性复合体-I蛋白表达高于对照妊娠。绵羊SCNT妊娠母胎界面存在不利的免疫环境。
