Hu Guo-Jun, Dong Ya-Feng, Zhang Zun-Ping, Fan Xu-Dong, Ren Fang, Li Zhengnan
Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, 125100, PR China.
Arch Virol. 2017 Aug;162(8):2397-2402. doi: 10.1007/s00705-017-3384-3. Epub 2017 Apr 25.
Two primer pairs were used to detect apple stem pitting virus (ASPV) using a reverse transcription (RT)-PCR test. 82 out of the 141 randomly collected samples, from ten orchards in five provinces and regions of China, tested positive. In the positive samples forty-five (55%) were infected by ASPV and two other viruses. The full coat protein (CP) and the triple gene block (TGB) gene 1, 2 and 3 of partial ASPV isolates were subsequently cloned. The nucleotide and amino acid identities of 39 CP sequence variants from 31 Chinese apple samples were compared with that of previously reported ASPV isolates and were 67.4-96.0% and 68.4-97.7%, respectively. All ASPV sequence variants from Chinese apples separated into two clades with CP- and TGB-based phylogenetic trees, whilst the grouping of TGB2 and TGB3 trees was the same. Three recombinants (FS06-2, X5-2, and XLF-C-2) for CP and six (TH2-5, X8-2, FS05-2, X6-2 and XLF-A-1) recombinants for TGB were identified from the Chinese apple isolates. Two recombinants were found in the TGB sequence of isolate XLF-A-1. The results presented here may assist in the development of a more comprehensive screening tool for apple viruses.
使用两对引物通过逆转录（RT）-PCR试验检测苹果茎痘病毒（ASPV）。在中国五个省和地区的十个果园随机采集的141个样本中，有82个检测呈阳性。在阳性样本中，45个（55%）感染了ASPV和另外两种病毒。随后克隆了部分ASPV分离株的完整外壳蛋白（CP）以及三基因块（TGB）基因1、2和3。将来自31个中国苹果样本的39个CP序列变体的核苷酸和氨基酸同一性与先前报道的ASPV分离株进行比较，分别为67.4 - 96.0%和68.4 - 97.7%。基于CP和TGB的系统发育树将来自中国苹果的所有ASPV序列变体分为两个进化枝，而TGB2和TGB3树的分组相同。从中国苹果分离株中鉴定出3个CP重组体（FS06 - 2、X5 - 2和XLF - C - 2）和6个TGB重组体（TH2 - 5、X8 - 2、FS05 - 2、X6 - 2和XLF - A - 1）。在分离株XLF - A - 1的TGB序列中发现了两个重组体。本文给出的结果可能有助于开发一种更全面的苹果病毒筛选工具。
