[Effect of Stromal Cell Co-culture on Drug Resistance of Acute Myeloid Leukemia Cells and its Mechanism].
作者信息
Yue Han, Liu Li-Ying, Wang Xiao-Qian
机构信息
Department of Hematology and Oncology, Zhenzhou Municipal First People's Hospital, Zhengzhou 450052, Henan Province, China. E-mail:
Department of Hematology and Oncology, Zhenzhou Municipal First People's Hospital, Zhengzhou 450052, Henan Province, China.
出版信息
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2017 Apr;25(2):382-386. doi: 10.7534/j.issn.1009-2137.2017.02.013.
OBJECTIVE
To study the effect of co-culture of stromal cells and acute myeloid leukemia(AML) cells on drug resistance of AML cells and its mechanism.
METHODS
Stromal cells were co-cultured with acute myeloid leukemia cell HL-60 and then were treated with DNR, HHT and Ara-C for observing the sensitivity of HL-60 cells to drugs after incubation with HS-5. At the same time, the the inhibitor LY294002 of PI3K/AKT signaling pathway was used to treat the cells, so as to explore whether the changes of HL-60 sensitivity is associated with the activation of PI3K/AKT signal pathway after co-culture of cells.
RESULTS
The statistical results of HL-60 cell inhibition rate showed that the HL-60 cell sensitivity to drugs was decreased after incubation with HS-5, the mRNA quantitation and immunblot detection showed that PI3K/AKT signaling pathway was activated after co-culture of HL-60 cells with HS-5 cells, in addition the CCND1, FOXO1, PTEN and other important genes were also changed significantly.
CONCLUSION
After co-culture of HL-60 cells with HS-5, some important molecules of PI3K/AKT signal pathway are changed, such as CCND1,FOXO1, PTEN, finally leading to the change of HL-60 cell sensitivity to drugs.
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