Sun Xiuxiu, Baldwin Elizabeth, Plotto Anne, Narciso Jan, Ference Christopher, Ritenour Mark, Harrison Ken, Gangemi Joseph, Bai Jinhe
USDA, ARS, USHRL; Indian River Research and Education Center, University of Florida.
USDA, ARS, USHRL.
J Vis Exp. 2017 Apr 7(122):55400. doi: 10.3791/55400.
A controlled-release chlorine dioxide (ClO2) pouch was developed by sealing a slurry form of ClO2 into semipermeable polymer film; the release properties of the pouch were monitored in containers with or without fruit. The pouch was affixed to the inside of a perforated clamshell containing grape tomatoes, and the effect on microbial population, firmness, and weight loss was evaluated during a 14 day storage period at 20 °C. Within 3 days, the ClO2 concentration in the clamshells reached 3.5 ppm and remained constant until day 10. Thereafter, it decreased to 2 ppm by day 14. The ClO2 pouch exhibited strong antimicrobial activity, reducing Escherichia coli populations by 3.08 log CFU/g and Alternaria alternata populations by 2.85 log CFU/g after 14 days of storage. The ClO2 treatment also reduced softening and weight loss and extended the overall shelf life of the tomatoes. Our results suggest that ClO2 treatment is useful for extending the shelf life and improving the microbial safety of tomatoes during storage without impairing their quality.
通过将二氧化氯(ClO₂)的浆液形式密封在半透性聚合物薄膜中来制备控释二氧化氯袋;在有或没有水果的容器中监测该袋子的释放特性。将该袋子固定在装有葡萄番茄的穿孔蛤壳内部,并在20℃下储存14天期间评估其对微生物数量、硬度和失重的影响。在3天内,蛤壳内的ClO₂浓度达到3.5 ppm,并在第10天之前保持恒定。此后,到第14天它降至2 ppm。ClO₂袋表现出很强的抗菌活性,储存14天后,大肠杆菌数量减少3.08 log CFU/g,链格孢数量减少2.85 log CFU/g。ClO₂处理还减少了软化和失重,并延长了番茄的整体货架期。我们的结果表明,ClO₂处理有助于在不损害番茄品质的情况下延长其货架期并提高储存期间的微生物安全性。