Optimization of milk odd and branched-chain fatty acids analysis by gas chromatography using an extremely polar stationary phase.

Odd and branched-chain fatty acids (OBCFA) are of interest, since they have bioactive properties and could be regarded biomarkers of ruminant fat intake. An accurate analysis of the individual OBCFA in milk by gas chromatography (GC) is not easy due to milk fat complexity. The availability of ionic liquid stationary phases as SLB-IL111 can be a useful tool to discriminate OBCFA from other milk FA eluting in the same chromatographic regions. The elution behavior of OBCFA on SLB-IL111 was evaluated based on different GC oven temperature programs. All programs assayed discriminated 11:0, iso 13:0, anteiso 13:0, iso 15:0, anteiso 15:0, 15:0 and iso 17:0. Using an initial temperature of 150°C for 1h, 13:0 and iso 16:0 were separated from trans-12:1 and 13-14:1, respectively, whereas iso 18:0 was discriminated from cis-16:1 isomers. 17:0 and 21:0 were well resolved only when an initial GC temperature of 160°C was applied.