Fan Jixiang, Chen Dongyun, Li Najun, Xu Qingfeng, Li Hua, He Jinghui, Lu Jianmei
College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou, 215123, China.
Sci Rep. 2017 Apr 28;7(1):1271. doi: 10.1038/s41598-017-01539-0.
Microorganism immobilization has attracted great attention as a traditional method to overcome aqueous organic wastes containing N, N-dimethylformamide (DMF). In this approach, graphene oxide was modified with functional polymer firstly to obtain micro-composites material (PGO), and then the prepared composites were deposited on the surface of copper mesh (CM) to block the meshes and CM@PGO was achieved. Moreover, cage-shaped model was designed based on CM@PGO and P. denitrificans was packed inside the cage for batch experiments. This strategy could enrich the local concentration of DMF due to the formation of hydrogen bonds with the oxygen-containing groups from PGO and the character of bacteria in captivity could also contribute to the process of degradation. Results showed that the approach could remove DMF more efficiently about 15% compared with free microorganism and presented excellent cycling performance. Meantime, physical adsorption and chemical adsorption were both contributed to the process of PGO adsorption, and the adsorption isotherm fits Langmuir model well, furthermore, the theoretical maximum of adsorption ability calculated through Langmuir model is 95 mg/g. In other words, this cage-shaped CM@PGO provided a facile platform for treating various wastewaters by altering the species of packed microorganisms, which exhibited considerable prospects for wastewater treatment.
微生物固定化作为一种处理含N，N-二甲基甲酰胺（DMF）的水性有机废物的传统方法，已引起广泛关注。在该方法中，首先用功能聚合物对氧化石墨烯进行改性以获得微复合材料（PGO），然后将制备的复合材料沉积在铜网（CM）表面以堵塞网孔，从而得到CM@PGO。此外，基于CM@PGO设计了笼状模型，并将反硝化假单胞菌封装在笼内进行分批实验。由于与PGO中的含氧基团形成氢键，该策略可以提高DMF的局部浓度，并且被捕获细菌的特性也有助于降解过程。结果表明，与游离微生物相比，该方法去除DMF的效率更高，约为15%，并且具有优异的循环性能。同时，物理吸附和化学吸附都对PGO的吸附过程有贡献，吸附等温线很好地符合朗缪尔模型，此外，通过朗缪尔模型计算的理论最大吸附能力为95mg/g。换句话说，这种笼状CM@PGO通过改变封装微生物的种类为处理各种废水提供了一个简便的平台，在废水处理方面展现出可观的前景。
