从牛脑中快速纯化肌醇单磷酸磷酸酶
Rapid purification of inositol monophosphate phosphatase from beef brain.
作者信息
Meek J L, Rice T J, Anton E
机构信息
Central Research and Development Department, E. I. Dupont de Nemours & Co., Wilmington, DE 19898.
出版信息
Biochem Biophys Res Commun. 1988 Oct 14;156(1):143-8. doi: 10.1016/s0006-291x(88)80816-7.
A procedure is described for preparation of homogeneous inositol monophosphate phosphatase (EC 3.1.3.25) from beef brain in less than 2 days with an overall recovery of 15-25%. This enzyme, an essential part of the inositol phospholipid cycle in brain, is a proposed site of action of lithium ions in manic-depressive disorders. The major purification steps are: a) removal of most interfering protein by heat denaturation at 75 degrees C for 1 h, b) separation by anion exchange at a pH (6.0) near the enzyme's pI (4.9), and c) adsorption of most remaining impurities on a Procion Red affinity column.
本文描述了一种在不到两天的时间内从牛脑中制备均一肌醇单磷酸磷酸酶（EC 3.1.3.25）的方法，总回收率为15%-25%。这种酶是脑中肌醇磷脂循环的重要组成部分，被认为是锂离子在躁郁症中的作用位点。主要的纯化步骤如下：a）通过在75℃下加热变性1小时去除大部分干扰蛋白；b）在接近酶的pI（4.9）的pH值（6.0）下进行阴离子交换分离；c）在Procion Red亲和柱上吸附大部分残留杂质。
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