Vliegen Gwendolyn, Raju Tom K, Adriaensen Dirk, Lambeir Anne-Marie, De Meester Ingrid
Laboratory of Medical Biochemistry, Department of Pharmaceutical Sciences, University of Antwerp, 2610 Wilrijk, Belgium.
Laboratory of Cell Biology and Histology, Department of Veterinary Sciences, University of Antwerp, 2610 Wilrijk, Belgium.
Ann Transl Med. 2017 Mar;5(6):130. doi: 10.21037/atm.2017.03.36.
The pathophysiology of lung diseases is very complex and proteolytic enzymes may play a role or could be used as biomarkers. In this review, the literature was searched to make an overview of what is known on the expression of the proline-specific peptidases dipeptidyl peptidase (DPP) 4, 8, 9, prolyl oligopeptidase (PREP) and fibroblast activation protein α (FAP) in the healthy and diseased lung. Search terms included asthma, chronic obstructive pulmonary disease (COPD), lung cancer, fibrosis, ischemia reperfusion injury and pneumonia. Knowledge on the loss or gain of protein expression and activity during disease might tie these enzymes to certain cell types, substrates or interaction partners that are involved in the pathophysiology of the disease, ultimately leading to the elucidation of their functional roles and a potential therapeutic target. Most data could be found on DPP4, while the other enzymes are less explored. Published data however often appear to be conflicting, the applied methods divers and the specificity of the assays used questionable. In conclusion, information on the expression of the proline-specific peptidases in the healthy and diseased lung is lacking, begging for further well-designed research.
肺部疾病的病理生理学非常复杂，蛋白水解酶可能发挥作用或可作为生物标志物。在本综述中，检索了相关文献，以概述脯氨酸特异性肽酶二肽基肽酶（DPP）4、8、9、脯氨酰寡肽酶（PREP）和成纤维细胞活化蛋白α（FAP）在健康和患病肺组织中的表达情况。检索词包括哮喘、慢性阻塞性肺疾病（COPD）、肺癌、纤维化、缺血再灌注损伤和肺炎。了解疾病期间蛋白质表达和活性的丧失或增加可能会将这些酶与疾病病理生理学中涉及的某些细胞类型、底物或相互作用伙伴联系起来，最终有助于阐明它们的功能作用和潜在的治疗靶点。关于DPP4的研究数据最多，而对其他酶的研究较少。然而，已发表的数据往往相互矛盾，所应用的方法多种多样，所用检测方法的特异性也值得怀疑。总之，目前缺乏关于脯氨酸特异性肽酶在健康和患病肺组织中表达的信息，需要进一步开展精心设计的研究。
