大鼠心脏迷走神经节中β-肾上腺素能受体的定量放射自显影术。

Quantitative autoradiography of beta-adrenoceptors in the cardiac vagus ganglia of the rat.

作者信息

Saito K, Potter W Z, Saavedra J M

机构信息

Unit of Preclinical Neuropharmacology, National Institute of Mental Health, Bethesda, MD 20892.

出版信息

Eur J Pharmacol. 1988 Aug 24;153(2-3):289-93. doi: 10.1016/0014-2999(88)90617-6.

DOI:10.1016/0014-2999(88)90617-6

PMID:2846320

Abstract

We studied beta-adrenoceptors by quantitative autoradiography in the cardiac vagus ganglia of the rat, after incubation of tissue sections with [125I]iodocyanopindolol. The cardiac vagus ganglia presented a high concentration of a single population of high affinity binding sites (Bmax 109 +/- 10 fmol/mg protein; Kd 64 +/- 5 pM). Displacement studies with the beta 1-selective adrenoceptor antagonist CGP 20712 A and the beta 2-selective adrenoceptor antagonist ICI 118,551 indicated that most (80 to 90%) of the binding sites are of the beta 2-type.

摘要

在用[125I]碘氰吲哚洛尔孵育组织切片后，我们通过定量放射自显影术研究了大鼠心脏迷走神经节中的β-肾上腺素能受体。心脏迷走神经节呈现出单一高亲和力结合位点群体的高浓度（Bmax为109±10 fmol/mg蛋白质；Kd为64±5 pM）。用β1选择性肾上腺素能受体拮抗剂CGP 20712 A和β2选择性肾上腺素能受体拮抗剂ICI 118,551进行的置换研究表明，大多数（80%至90%）结合位点为β2型。