劳氏肉瘤病毒MA gag区域中假定的反式激活因子对于细胞转化并非必需。
The putative trans-activator in the MAgag region of Rous sarcoma virus is not required for cell transformation.
作者信息
Dutta A, Dorai T, Hanafusa H
机构信息
Rockefeller University, New York, New York 10021-6399.
出版信息
J Virol. 1988 Dec;62(12):4767-9. doi: 10.1128/JVI.62.12.4767-4769.1988.
Abstract
A stop codon created by oligonucleotide-directed mutagenesis in the proposed transcriptional trans-activator of Rous sarcoma virus (S. Broome and W. Gilbert, Cell 40:537-546, 1985) which truncated the trans-activator by half did not alter the transforming activity or the replication of the virus in primary chicken embryo fibroblasts. This result proves that this trans-activator is not essential for transformation of primary cells by Rous sarcoma virus.
摘要
通过寡核苷酸定向诱变在劳氏肉瘤病毒的拟转录反式激活因子中产生的一个终止密码子（S. 布鲁姆和W. 吉尔伯特，《细胞》40:537 - 546, 1985），该终止密码子使反式激活因子截短了一半，但并未改变病毒在原代鸡胚成纤维细胞中的转化活性或复制。这一结果证明，该反式激活因子对于劳氏肉瘤病毒转化原代细胞并非必不可少。
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