Charng Yuh-Chyang, Hsu Lung-Hsin, Liu Li-Yu Daisy
Department of Agronomy, National Taiwan University, Taipei, Taiwan.
Evol Bioinform Online. 2017 Feb 23;13:1176934317690410. doi: 10.1177/1176934317690410. eCollection 2017.
In exonization events, may provide donor and/or acceptor sites for splicing after inserting into genes and be incorporated into new transcripts with new exon(s). In this study, the protein variants of exonization yielding additional functional profile(s) were studied. Unlike exonization, which creates new profiles mostly by incorporating flanking intron sequences with the message, exonization additionally creates new profiles through the presence or absence of messages. The number of unique functional profiles harboring messages is 1.3-fold more than that of functional profiles without messages. The highly similar 11 protein isoforms at a single insertion site also contribute to proteome complexity enrichment by exclusively creating new profiles. Particularly, exonization produces 459 unique profiles, of which 129 cannot be built by . We thus conclude that and are independent but synergistic in their capacity to enrich proteome complexity through exonization.
在外显子化事件中，插入基因后可能为剪接提供供体和/或受体位点，并与新的外显子一起被纳入新的转录本中。在本研究中，对产生额外功能谱的外显子化的蛋白质变体进行了研究。与主要通过将侧翼内含子序列与信息整合来创建新谱的外显子化不同，外显子化还通过信息的存在与否额外创建新谱。含有信息的独特功能谱的数量比不含信息的功能谱多1.3倍。在单个插入位点高度相似的11种蛋白质异构体也通过专门创建新谱来促进蛋白质组复杂性的富集。特别是，外显子化产生了459种独特的谱，其中129种不能由构建。因此，我们得出结论，和在通过外显子化富集蛋白质组复杂性的能力上是独立但协同的。
