Fis通过影响PA14菌株中 的转录来调节III型分泌系统。
Fis Regulates Type III Secretion System by Influencing the Transcription of in Strain PA14.
作者信息
Deng Xuan, Li Mei, Pan Xiaolei, Zheng Ruiping, Liu Chang, Chen Fei, Liu Xue, Cheng Zhihui, Jin Shouguang, Wu Weihui
机构信息
State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai UniversityTianjin, China.
State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai UniversityTianjin, China.
出版信息
Front Microbiol. 2017 Apr 19;8:669. doi: 10.3389/fmicb.2017.00669. eCollection 2017.
Fis is a versatile DNA binding protein in bacteria. It has been demonstrated in multiple bacteria that Fis plays crucial roles in regulating bacterial virulence factors and optimizing bacterial adaptation to various environments. However, the role of Fis in virulence as well as gene regulation remains largely unknown. Here, we found that Fis was required for the virulence of in a murine acute pneumonia model. Transcriptome analysis revealed that expression of T3SS genes, including master regulator ExsA, was defective in a ::Tn mutant. We further demonstrate that the continuous transcription of , and driven by the promoter was required for the activation of T3SS. Fis was found to specifically bind to the - intergenic region and plays an essential role in the transcription elongation from to . Therefore, we found a novel role of Fis in the regulation of expression.
Fis是细菌中一种多功能的DNA结合蛋白。在多种细菌中已证实，Fis在调节细菌毒力因子和优化细菌对各种环境的适应性方面发挥着关键作用。然而，Fis在毒力以及基因调控方面的作用仍 largely未知。在此，我们发现在小鼠急性肺炎模型中，Fis是 毒力所必需的。转录组分析显示，包括主调节因子ExsA在内的III型分泌系统（T3SS）基因的表达在一个 ::Tn突变体中存在缺陷。我们进一步证明，由 启动子驱动的 、 和 的持续转录是激活T3SS所必需的。发现Fis特异性结合到 - 基因间区域，并在从 到 的转录延伸中起重要作用。因此，我们发现了Fis在调节 表达方面的新作用。
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