原核生物整合膜蛋白在大肠杆菌中的表达
Expression of Prokaryotic Integral Membrane Proteins in E. coli.
作者信息
Love James D
机构信息
Department of Biochemistry, Albert Einstein College of Medicine at Yeshiva University, Bronx, NY, USA.
ATUM, 37950 Central Court, Newark, CA, 94560, USA.
出版信息
Methods Mol Biol. 2017;1586:265-278. doi: 10.1007/978-1-4939-6887-9_17.
PMID:28470611
Abstract
Production of prokaryotic membrane proteins for structural and functional studies in E. coli can be parallelized and miniaturized. All stages from cloning, expression, purification to detergent selection can be investigated using high-throughput techniques to rapidly and economically find tractable targets.
摘要
在大肠杆菌中生产用于结构和功能研究的原核膜蛋白可以实现并行化和小型化。从克隆、表达、纯化到去污剂选择的所有阶段都可以使用高通量技术进行研究，以快速、经济地找到易于处理的目标。
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