白三烯A4在人T细胞系中转化为白三烯B4 。

Leukotriene A4, conversion to leukotriene B4 in human T-cell lines.

作者信息

Fu J Y, Medina J F, Funk C D, Wetterholm A, Rådmark O

机构信息

Dept. of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden.

出版信息

Prostaglandins. 1988 Aug;36(2):241-8. doi: 10.1016/0090-6980(88)90310-3.

DOI:10.1016/0090-6980(88)90310-3

PMID:2847252

Abstract

Human T-cell lines (HSB, MOLT-4 and CCRF-CEM) produced leukotriene B4 when incubated with leukotriene A4. The product was characterized by chromatographic properties, UV-spectroscopy and gas chromatography mass spectrometry. About 10 pmol of leukotriene B4 was obtained per 10(6) cells. When incubated with arachidonic acid plus the calcium ionophore A23187 however, no leukotriene B4 was found, indicating that the T-cell lines lack 5-lipoxygenase yet contain LTA4 hydrolase.

摘要

人T细胞系（HSB、MOLT - 4和CCRF - CEM）与白三烯A4一起孵育时会产生白三烯B4。该产物通过色谱特性、紫外光谱和气相色谱 - 质谱进行表征。每10⁶个细胞可获得约10皮摩尔的白三烯B4。然而，当与花生四烯酸加钙离子载体A23187一起孵育时，未发现白三烯B4，这表明T细胞系缺乏5 - 脂氧合酶，但含有白三烯A4水解酶。