Kalita Prasanta, Dasgupta Anshuman, Gupta Shalini
Department of Chemical Engineering, Indian Institute of Technology, Hauz Khas, 110016, Delhi, India.
Department of Nanomedicine and Theranostics, Institute for Experimental Molecular Imaging, RWTH Aachen University Clinic, Aachen, Germany.
Methods Mol Biol. 2017;1600:133-142. doi: 10.1007/978-1-4939-6958-6_13.
Bloodstream bacterial infections are known to illicit a systemic immune response that can lead to multiorgan failure and septic shock. The current endotoxin identification techniques in serum are expensive and elaborate requiring bulky benchtop instrumentation. We demonstrate a new route for endotoxin detection in which lipopolysaccharides (LPS) in solution are entrapped using C-18 silane-functionalized glass slides and tagged with polymyxin B sulfate (PMB) drug-conjugated gold nanoparticles. The signal from the particles is further amplified via the silver reduction approach to yield concentration-dependent colorimetric spots visible to the bare eye. The method is rapid, reliable, and cost-effective and fulfills an urgent unmet need in the healthcare industry for early septicemia diagnosis.
已知血流细菌感染会引发全身性免疫反应，进而导致多器官功能衰竭和感染性休克。目前血清中内毒素的鉴定技术昂贵且复杂，需要大型台式仪器。我们展示了一种新的内毒素检测方法，其中溶液中的脂多糖（LPS）被C-18硅烷功能化玻片捕获，并用硫酸多粘菌素B（PMB）药物偶联的金纳米颗粒进行标记。通过银还原方法进一步放大颗粒的信号，以产生肉眼可见的浓度依赖性比色斑点。该方法快速、可靠且具有成本效益，满足了医疗行业对早期败血症诊断迫切未满足的需求。
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