Salihoglu Murat, Dogru Salim, Cesmeci Enver, Caliskan Halil, Kurt Onuralp, Kuçukodaci Zafer, Gungor Atila
GATA Haydarpasa Training Hospital, Department of Otorhinolaryngology, Istanbul, Turkey.
Efes Otolaryngology Branch Center, Department of Otorhinolaryngology, Izmir, Turkey.
Braz J Otorhinolaryngol. 2018 May-Jun;84(3):332-337. doi: 10.1016/j.bjorl.2017.03.010. Epub 2017 Apr 22.
Boric acid, which has antiseptic and acidic properties, is used to treat external and middle ear infections. However, we have not found any literature about the effect of boric acid powder on middle ear mucosa and inner ear.
The purpose of this study is to investigate possible ototoxic effects of boric acid powder on cochlear outer hair cell function and histological changes in middle ear mucosa in a rat animal model.
Twenty healthy, mature Wistar albino rats were used in this study. The rats were divided into two groups, Group A and Group B, each of which consisted of 10 rats. Initially, the animals in each group underwent distortion product otoacoustic emissions testing of their right and left ears. After the first distortion product otoacoustic emissions test, a surgical microscope was used to make a small perforation in both ears of the rats in each group, and a second distortion product otoacoustic emissions test was used to measure both ears in all of the rats. Boric acid powder was applied to the right middle ear of the rats using tympanic membrane perforation, and the distortion product otoacoustic emissions were measured immediately after the boric acid powder application. The histological changes and distortion product otoacoustic emissions were evaluated three days later in Group A and 40 days later in Group B.
No significant differences were found at all of the distortion product otoacoustic emissions frequencies. In Group A, mild inflammation of the middle ear mucosa was found on the third day after boric acid powder application. In Group B, boric acid powder caused mild inflammatory changes on the 40th day, which declined over time. Those changes did not lead to significant fibrosis within the mucosa.
In rats, boric acid powder causes mild inflammation in middle ear mucosa and it has no ototoxic effects on cochlear outer hair cell function in the inner ear of rats.
具有防腐和酸性特性的硼酸用于治疗外耳和中耳感染。然而，我们尚未发现任何有关硼酸粉对中耳黏膜和内耳影响的文献。
本研究旨在探讨在大鼠动物模型中硼酸粉对耳蜗外毛细胞功能及中耳黏膜组织学变化可能产生的耳毒性作用。
本研究使用了20只健康、成熟的Wistar白化大鼠。将大鼠分为A组和B组，每组10只。最初，对每组大鼠的左右耳进行畸变产物耳声发射测试。在首次畸变产物耳声发射测试后，使用手术显微镜在每组大鼠的双耳制造一个小穿孔，并再次进行畸变产物耳声发射测试以测量所有大鼠的双耳。通过鼓膜穿孔将硼酸粉应用于大鼠的右中耳，并在应用硼酸粉后立即测量畸变产物耳声发射。A组在三天后、B组在40天后评估组织学变化和畸变产物耳声发射。
在所有畸变产物耳声发射频率上均未发现显著差异。在A组中，应用硼酸粉后第三天发现中耳黏膜有轻度炎症。在B组中，硼酸粉在第40天引起轻度炎症变化，且随时间推移有所减轻。这些变化未导致黏膜内出现明显纤维化。
在大鼠中，硼酸粉会引起中耳黏膜轻度炎症，且对大鼠内耳的耳蜗外毛细胞功能无耳毒性作用。
