慢性乙型肝炎感染患者循环中Th9细胞频率降低。
Decreased frequency of circulating Th9 cells in patients with chronic hepatitis B infection.
作者信息
Cui Meilan, Lv Ying, Lu Jianhua, Zhang Wei, Duan Yuanyuan, Huang Yan, Yang Lei, Li Man, Liu Wenxuan, Liu Dianwu, Yan Huimin
机构信息
Clinical Research Center, Shijiazhuang Fifth Hospital, Shijiazhuang, Hebei, China.
Graduate College of Hebei Medical University, Hebei Medical University, Shijiazhuang, Hebei, China.
出版信息
J Clin Lab Anal. 2018 Feb;32(2). doi: 10.1002/jcla.22246. Epub 2017 May 8.
BACKGROUND
Chronic hepatitis B (CHB) is one of the major infectious diseases in which CD4+ T helper (Th) cells play a crucial role. Th9 cells are a distinct subset of CD4+ Th cells with important physiological functions.
OBJECTIVE
The study aimed to assess the potential involvement of Th9 cells in the inadequate immune response leading to chronic HBV infection.
PATIENTS AND METHODS
Peripheral blood samples were collected from 22 CHB patients and 16 healthy controls (HC). The frequencies of circulating Th9, Tc9, Th1, and Tc1 cells were determined by flow cytometry. Serum levels of IL-9 and IL-10 were analyzed by ELISA. Serum biochemical indices of liver function were measured using an automated analyzers. Serum HBV DNA loads were analyzed by real-time PCR. The potential association of the frequency of Th9, Tc9, Th1 or Tc1 cells with clinical parameters was assessed.
RESULTS
The frequency of circulating Th9 cells was significantly lower in CHB patients than those in HC. However, no significant differences in the frequency of Tc9, Th1 or Tc1 cells were observed between the two groups. The percentages of Th9 cells, but not Tc9 cells, were negatively correlated with HBV DNA loads, whereas the percentages of Tc1 cells were positively correlated with viral loads in CHB patients. Moreover, there was a positive correlation between serum levels of IL-9 and IL-10 and HBV DNA loads in patients with chronic HBV infection.
CONCLUSION
The depletion of Th9 cells is associated with the development of chronic HBV infection.
背景
慢性乙型肝炎（CHB）是主要的传染病之一，其中CD4 +辅助性T（Th）细胞发挥着关键作用。Th9细胞是具有重要生理功能的CD4 + Th细胞的一个独特亚群。
目的
本研究旨在评估Th9细胞在导致慢性HBV感染的免疫反应不足中的潜在作用。
患者和方法
收集22例CHB患者和16例健康对照（HC）的外周血样本。通过流式细胞术测定循环Th9、Tc9、Th1和Tc1细胞的频率。采用酶联免疫吸附测定法（ELISA）分析血清白细胞介素-9（IL-9）和白细胞介素-10（IL-10）水平。使用自动分析仪测量血清肝功能生化指标。通过实时聚合酶链反应（PCR）分析血清HBV DNA载量。评估Th9、Tc9、Th1或Tc1细胞频率与临床参数之间的潜在关联。
结果
CHB患者循环Th9细胞频率显著低于健康对照。然而，两组之间Tc9、Th1或Tc1细胞频率无显著差异。CHB患者中，Th9细胞百分比与HBV DNA载量呈负相关，而Tc9细胞百分比与病毒载量无此相关性，Tc1细胞百分比与病毒载量呈正相关。此外，慢性HBV感染患者血清IL-9和IL-10水平与HBV DNA载量呈正相关。
结论
Th9细胞的耗竭与慢性HBV感染的发生有关。
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