Department of Pediatrics, Yantaishan Hospital, Yantai, China.
Eur Rev Med Pharmacol Sci. 2017 Apr;21(8):1904-1910.
The purpose of this study was to elucidate the possible beneficial effects of pachymic acid (PA) on acute lung injury (ALI) in a rat model of sepsis.
A rat model of sepsis induced by cecal ligation and puncture (CLP) was used. Rats were randomly divided into five groups: sham, CLP, CLP + PA 1 mg/kg, CLP + PA 5 mg/kg, CLP + PA 10 mg/kg. CLP + PA groups received PA by intraperitoneal injection daily for consecutive 3 days, respectively, and the rats in sham and CLP groups were given equivalent volume of olive oil. We killed the animals 12 h after CLP and collected blood samples to determine PaO2, PaCO2, tumor necrosis factor-alpha (TNF-a), interleukin-1β (IL-1β) and IL-6. Lung samples were taken for wet/dry weight ratios and histologic assessment. Meanwhile, the levels of lung tissue myeloperoxidase (MPO), malondialdehyde (MDA) and superoxide dismutase (SOD) were determined.
The results revealed that PA treatment significantly improved the survival of septic rats and attenuated CLP-induced ALI. In PA-treated rats, the wet/dry weight ratios and the serum levels of TNF-a, IL-1β and IL-6 were down-regulated compared with the CLP group. PA also markedly decreased MDA and MPO contents and increased SOD level.
These findings indicate that PA administration ameliorates ALI in a rat model of sepsis induced by CLP.
本研究旨在阐明棕矢车菊酸（PA）对脓毒症大鼠急性肺损伤（ALI）可能的有益作用。
采用盲肠结扎穿孔（CLP）法建立脓毒症大鼠模型。大鼠随机分为 5 组：假手术组、CLP 组、CLP+PA1mg/kg 组、CLP+PA5mg/kg 组、CLP+PA10mg/kg 组。CLP+PA 组分别每日腹腔注射 PA，连续 3 天，假手术和 CLP 组给予等体积橄榄油。CLP 后 12h 处死动物，采集血样测定 PaO2、PaCO2、肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）。取肺组织测定湿/干重比和组织学评估。同时，测定肺组织髓过氧化物酶（MPO）、丙二醛（MDA）和超氧化物歧化酶（SOD）的水平。
PA 治疗显著提高了脓毒症大鼠的存活率，并减轻了 CLP 诱导的 ALI。与 CLP 组相比，PA 治疗组大鼠的湿/干重比以及血清 TNF-α、IL-1β和 IL-6 水平降低。PA 还显著降低 MDA 和 MPO 含量，增加 SOD 水平。
这些发现表明，PA 给药可改善 CLP 诱导的脓毒症大鼠的 ALI。
