Department of Chemical Engineering, Imperial College London, London SW7 2AZ, United Kingdom; Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Bioprocessing and Biomanufacturing Research Centre, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Bioprocessing and Biomanufacturing Research Centre, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Food Chem. 2017 Oct 1;232:245-252. doi: 10.1016/j.foodchem.2017.03.102. Epub 2017 Mar 23.
Bacteriocin is an important peptide which can be used as an anti-microbial agent in food. However, simpler and more cost-effective purification methods need to be developed compared to chromatography to enhance its commercial viability. Surfactant precipitation was employed for the first time to purify bacteriocin-like inhibitory substance (BLIS) from a fermentation broth of Pediococcus acidilactici Kp10, and the amount precipitated was investigated as a function of anionic surfactant (AOT) concentration, and pH. Protein recovery from the precipitate was accomplished using solvent extraction, and solvent type, NaCl concentration, and ionic strength of the final solution were optimised. Optimal conditions were; 1.05mM of AOT at pH 4 for precipitation, and acetone extraction (with 1mM NaCl), which resulted in an 86.3% yield, and 53.8 purification factor. This study highlighted the fact that surfactant precipitation can be used as a primary recovery method for BLIS from a complex fermentation broth.
细菌素是一种重要的肽类物质，可用作食品中的抗菌剂。然而，与色谱法相比，需要开发更简单、更具成本效益的纯化方法，以提高其商业可行性。本研究首次采用表面活性剂沉淀法从戊糖片球菌 Kp10 的发酵液中纯化细菌素样抑制物质（BLIS），并研究了沉淀量与阴离子表面活性剂（AOT）浓度和 pH 的关系。通过溶剂萃取从沉淀物中回收蛋白质，并优化了溶剂类型、最终溶液中的 NaCl 浓度和离子强度。最佳条件为：pH4 时 AOT 浓度为 1.05mM，采用丙酮萃取（含 1mM NaCl），产率为 86.3%，纯化倍数为 53.8。本研究结果表明，表面活性剂沉淀法可作为从复杂发酵液中提取 BLIS 的初步回收方法。
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