Perepechaeva M L, Seredina T A, Sidorova Y A, Pivovarova E N, Markel A L, Lyakhovich V V, Grishanova A Y
Institute of Molecular Biology and Biophysics, Novosibirsk 630117, Russia.
Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (SB RAS), Novosibirsk 630090, Russia.
Biomed Environ Sci. 2017 Apr;30(4):308-313. doi: 10.3967/bes2017.041.
We studied effects of nutrient quercetin on cytochromes' Р450 1А (CYP1A) activities (measured spectrofluorimetrically using 7-ethoxy-resorufin for CYP1A1 and 7-methoxy-resorufin for CYP1A2 as substrates), on mRNA levels (measured by RT-PCR), and on DNA-binding activities (evaluated by an electrophoretic mobility shift assay) of proteins regulating CYP1A expression in untreated and benzo(α)pyrene (BaP)-treated rats. Wistar rats received quercetin, BaP, or both once daily for 1-3 days. Quercetin did not influence CYP1A1 in untreated rats but inhibited BaP-mediated CYP1A induction on the transcriptional level decreasing positive input (AhR functional activity) and increasing negative input (AhRR/ARNT expression and Oct-1 and C/EBP functional activities).
我们研究了营养成分槲皮素对细胞色素P450 1A（CYP1A）活性（使用7-乙氧基-试卤灵作为CYP1A1的底物、7-甲氧基-试卤灵作为CYP1A2的底物，通过荧光分光光度法测定）、mRNA水平（通过逆转录聚合酶链反应测定）以及在未处理和苯并（α）芘（BaP）处理的大鼠中调节CYP1A表达的蛋白质的DNA结合活性（通过电泳迁移率变动分析评估）的影响。Wistar大鼠每日接受一次槲皮素、BaP或两者，持续1至3天。槲皮素对未处理大鼠的CYP1A1没有影响，但在转录水平上抑制了BaP介导的CYP1A诱导，降低了正向输入（芳烃受体功能活性）并增加了负向输入（芳烃受体阻遏物/芳烃核转运蛋白表达以及Oct-1和C/EBP功能活性）。
Zotero 插件