在发育中和成年性腺中的性别二态性表达表明，性腺体细胞衍生因子在牙鲆（Paralichthys olivaceus）性别分化过程中发挥着重要作用。

Sexually dimorphic expression in developing and adult gonads shows an important role of gonadal soma-derived factor during sex differentiation in olive flounder (Paralichthys olivaceus).

作者信息

Liu Yuezhong, Zhang Wei, Du Xinxin, Zhao Jun, Liu Xiaobing, Li Xiaojing, Zhang Quanqi, Wang Xubo

机构信息

Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, 266003 Qingdao, Shandong, China; Qingdao National Laboratory for Marine Science and Technology, China.

出版信息

Comp Biochem Physiol B Biochem Mol Biol. 2017 Aug;210:1-8. doi: 10.1016/j.cbpb.2017.05.003. Epub 2017 May 11.

DOI:10.1016/j.cbpb.2017.05.003

PMID:28502832

Abstract

Gonadal soma-derived factor (gsdf) is a new member of transforming growth factor beta (TGF-β) superfamily. As a teleost- and gonad-specific growth factor, gsdf has been indicated to play an important role in early germ cell development. However, little is known about its significance in germ cell development of olive flounder (Paralichthys olivaceus). In the present study, a 1338 bp gsdf gene was isolated from P. olivaceus for the first time. Bioinformatic analysis revealed that the genomic structure and synteny relationship of gsdf in teleosts were conserved. Quantitative real-time PCR (qRT-PCR) showed that gsdf expressed before sex gonadal differentiation, and the expression level increased rapidly after initiation of sex differentiation in males. In adult individuals, the expression of gsdf was higher in testis than that in ovary (P<0.01). In situ hybridization (ISH) indicated that gsdf mRNA was detected in the somatic cells of both males and females, and also in the cytoplasm of oocytes. These results suggested that gsdf might play an important role as initial switches to promote testis differentiation and participate in early germ cell development, such as proliferation and differentiation of spermatogonia and oogonia in P. olivaceus.

摘要

性腺体细胞衍生因子（gsdf）是转化生长因子β（TGF-β）超家族的一个新成员。作为一种硬骨鱼和性腺特异性生长因子，gsdf已被证明在早期生殖细胞发育中起重要作用。然而，关于其在牙鲆（Paralichthys olivaceus）生殖细胞发育中的意义知之甚少。在本研究中，首次从牙鲆中分离出一个1338 bp的gsdf基因。生物信息学分析表明，硬骨鱼中gsdf的基因组结构和共线性关系是保守的。实时定量PCR（qRT-PCR）显示，gsdf在性腺分化之前表达，在雄性开始性分化后表达水平迅速升高。在成年个体中，gsdf在睾丸中的表达高于卵巢（P<0.01）。原位杂交（ISH）表明，在雄性和雌性的体细胞以及卵母细胞的细胞质中均检测到gsdf mRNA。这些结果表明，gsdf可能作为促进睾丸分化的初始开关发挥重要作用，并参与早期生殖细胞发育，如牙鲆精原细胞和卵原细胞的增殖和分化。