与中华苦荬菜（Ixeridium chinense）畸形相关植原体的检测与鉴定。
Detection and identification of the phytoplasma associated with China ixeris (Ixeridium chinense) fasciation.
作者信息
Li Zheng-Nan, Liu Ping, Zhang Lei, Wu Yun-Feng
机构信息
State Key Laboratory of Crop Stress Biology for Arid Areas/Key Laboratory of Integrated Pest Management on Crop in Northwestern Loess Plateau, Ministry of Agriculture/Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi Province, P. R. China.
College of Forestry, Northwest A&F University, Yangling, Shaanxi, 712100, P. R. China.
出版信息
Bot Stud. 2013 Dec;54(1):52. doi: 10.1186/1999-3110-54-52. Epub 2013 Oct 31.
BACKGROUND
Phytoplasmas are always associated with symptoms in host plants such as stunting of stems, witches'-broom, yellowing of leaves, formation of sterile-deformed flowers, virescence and phyllody. Recently also symptom of fasciation was reported associated with phytoplasma presence. In the present work, China ixeris fasciation was observed associated with phytoplasmas in Guanzhong Area, Shaanxi, China.
RESULTS
Phytoplasma-like bodies were observed under transmission electron microscope in stem tissues of symptomatic samples. The 16S rRNA operon and tuf genes from phytoplasmas were amplified by PCR assays. Phylogenetic trees were calculated respectively based on sequences data of these two genes. The pattern of restriction fragment length polymorphism (RFLP) was generated via digesting the PCR products of 16S rRNA gene with eight restriction enzymes.
CONCLUSION
The presence of phytoplasma in China ixeris exhibiting fasciation symptom was confirmed by the results of TEM observation and PCR testing. Based on sequence data, phylogeny analysis and actual restriction fragment length polymorphism (RFLP) analysis, the associated phytoplasma was classified as related to 16SrI-C subgroup. This was the first record of phytoplasmas in China ixeris.
背景
植原体总是与寄主植物的症状相关联，如茎部矮化、扫帚状丛生、叶片黄化、不育变形花的形成、变绿和叶变花等。最近也有报道称，扁化症状与植原体的存在有关。在本研究中，在中国陕西关中地区观察到苦荬菜扁化与植原体有关。
结果
在有症状样本的茎组织中，通过透射电子显微镜观察到类植原体颗粒。通过PCR检测扩增了植原体的16S rRNA操纵子和tuf基因。分别基于这两个基因的序列数据构建了系统发育树。通过用8种限制性内切酶消化16S rRNA基因的PCR产物，产生了限制性片段长度多态性（RFLP）模式。
结论
通过透射电子显微镜观察和PCR检测结果，证实了表现出扁化症状的苦荬菜中存在植原体。基于序列数据、系统发育分析和实际的限制性片段长度多态性（RFLP）分析，相关植原体被归类为与16SrI-C亚组相关。这是苦荬菜中植原体的首次记录。
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