Huang Shih-Hung, Agrawal Dinesh Chandra, Wu Fang-Sheng, Tsay Hsin-Sheng
Department of Applied Chemistry, Chaoyang University of Technology, Taichung, Taiwan.
Department of Biology, Virginia Commonwealth University, Richmond, 23284-2012, VA, USA.
Bot Stud. 2014 Dec;55(1):56. doi: 10.1186/s40529-014-0056-4. Epub 2014 Jul 24.
Gentiana scabra Bunge commonly known as 'Long dan cao' in China has been used in traditional Chinese medicines for more than 2000 years. Dry roots and rhizome of the herb have been used for the treatment of inflammation, anorexia, indigestion and gastric infections. Iridoids and secoiridoids are the main bioactive compounds which attribute to the pharmacological properties of this plant. The species is difficult to mass propagate by seed due to the low percentage of germination and limited dormancy period. Wild populations in some locations are considered to be in the endangered category due to over exploitation.
In the present study, we report an efficient micropropagation system. Shoot apices of six weeks old in vitro grown G. scabra plants were used as explants for the in vitro propagation. Induction of multiple shoots (9.1/explant) was achieved on the culture of shoot apices on half strength Murashige and Skoog's basal medium (MSBM) containing 2.0 mg/L 6-benzylaminopurine (BA), 3% sucrose and 0.9% Difco agar. In vitro shoots induced profuse rooting on half strength of MSBM supplemented with 0.1 mg/L 1-naphthaleneacetic acid (NAA), 3% sucrose and 0.3% gelrite. A two-stage ventilation closure procedure during the in vitro culture, and transparent sachet technique enhanced the survival rate of G. scabra plantlets to 96% in the greenhouse. Tissue culture plants flowered after 5 months of transfer to pots.
A simple and an efficient in vitro propagation protocol of Gentiana scabra Bunge by optimizing the medium composition and ventilation closure treatments has been developed. The protocol can be very useful in germplasm conservation and commercial cultivation of G. scabra plants.
在中国通常被称为“龙胆草”的龙胆，已在传统中药中使用了2000多年。该草药的干燥根和根茎用于治疗炎症、食欲不振、消化不良和胃部感染。环烯醚萜类和裂环环烯醚萜类是赋予该植物药理特性的主要生物活性化合物。由于发芽率低和休眠期有限，该物种难以通过种子进行大规模繁殖。由于过度开发，一些地区的野生种群被认为处于濒危类别。
在本研究中，我们报道了一种高效微繁殖系统。六周龄的体外培养龙胆植株的茎尖用作体外繁殖的外植体。在含有2.0mg/L 6-苄基腺嘌呤（BA）、3%蔗糖和0.9% Difco琼脂的半强度Murashige和Skoog基础培养基（MSBM）上培养茎尖，可诱导多个芽（9.1个/外植体）。体外芽在添加了0.1mg/L 1-萘乙酸（NAA）、3%蔗糖和0.3%吉丽特的半强度MSBM上大量生根。体外培养期间的两阶段通气封闭程序和透明袋技术将龙胆植株在温室中的成活率提高到了96%。组培植株转移到花盆中5个月后开花。
通过优化培养基成分和通气封闭处理，开发了一种简单高效的龙胆体外繁殖方案。该方案对于龙胆种质保存和商业化种植可能非常有用。
