植物中染色体相关蛋白的免疫定位——原理与应用
Immunolocalization of chromosome-associated proteins in plants - principles and applications.
作者信息
de Paula Cristina Maria Pinto, Techio Vânia Helena
机构信息
Department of Biology, Federal University of Lavras, Lavras, Zip code 372000-000, Minas Gerais State, Brazil.
出版信息
Bot Stud. 2014 Dec;55(1):63. doi: 10.1186/s40529-014-0063-5. Epub 2014 Aug 5.
The use of the immunolocalization technique combined with cytogenetic and epigenetic studies is an indispensable tool and has contributed significantly to the analysis of the structure and function of chromosomes, since it can provide information about the spatial or temporal distribution of a given protein in the nucleus and chromosomes. Several chromosome-associated proteins in plant cells have already been identified by immunolocalization, such as histone and non-histone proteins and cell division-related protein (mitosis and meiosis). The principle of the immunolocalization technique in plants basically involves fixation and permeabilization of cells, the use of monoclonal or polyclonal antibodies attached to a signaling molecule, usually a fluorochrome and detection of the target molecule by using an epifluorescence microscope.
免疫定位技术与细胞遗传学和表观遗传学研究相结合的方法是一种不可或缺的工具，对染色体的结构和功能分析做出了重大贡献，因为它能够提供特定蛋白质在细胞核和染色体中的空间或时间分布信息。通过免疫定位，植物细胞中已经鉴定出了几种与染色体相关的蛋白质，如组蛋白、非组蛋白以及与细胞分裂相关的蛋白质（有丝分裂和减数分裂）。植物免疫定位技术的基本原理主要包括细胞固定和通透处理、使用与信号分子（通常是荧光染料）相连的单克隆或多克隆抗体，以及利用落射荧光显微镜检测目标分子。
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