Willsky G R, Dosch S F
Department of Biochemistry, State University of New York, Buffalo 14214.
Yeast. 1986 Jun;2(2):77-85. doi: 10.1002/yea.320020202.
Vanadium metabolism was studied in a wild type and respiratory-deficient strain of S. cerevisiae. Inhibition of growth by vanadate [V(+5)], vanadate accumulation, and conversion of medium vanadate [V(+5)] to both cell-associated and medium vanadyl [V(+4)] and vanadate [V(+5)] were compared. The growth of both the parental and respiratory-deficient strains was inhibited by vanadate at concentrations greater than or equal to 1 mM. Both parental and respiratory-deficient strains accumulated vanadate and converted medium vanadate to cellular vanadyl as detected using electron spin resonance (ESR). The accumulation of cell-associated vanadyl was correlated with the loss of medium vanadate in both strains using a chemical assay. In contrast, the respiratory-deficient strain showed a greater amount of a cell-associated vanadate compound, as detected with vanadium-51 nuclear magnetic resonance (51V-NMR), than the wild type strain or a representative respiratory-competent vanadate-resistant mutant. These data imply that mitochondrial function may be directly involved in vanadium metabolism.
在酿酒酵母的野生型和呼吸缺陷型菌株中研究了钒代谢。比较了钒酸盐[V(+5)]对生长的抑制作用、钒酸盐积累以及培养基中的钒酸盐[V(+5)]向细胞相关的和培养基中的钒酰[V(+4)]及钒酸盐[V(+5)]的转化。当钒酸盐浓度大于或等于1 mM时，亲本菌株和呼吸缺陷型菌株的生长均受到抑制。使用电子自旋共振（ESR）检测发现，亲本菌株和呼吸缺陷型菌株均积累钒酸盐并将培养基中的钒酸盐转化为细胞内的钒酰。通过化学分析可知，在两种菌株中，细胞相关钒酰的积累与培养基中钒酸盐的减少相关。相比之下，使用钒-51核磁共振（51V-NMR）检测发现，呼吸缺陷型菌株中与细胞相关的钒酸盐化合物的量比野生型菌株或具有代表性的呼吸功能正常的抗钒酸盐突变体更多。这些数据表明线粒体功能可能直接参与钒代谢。
