氘代氧化物在体外诱导变应性哮喘患者人嗜酸性粒细胞脱颗粒。

Deuterium-oxide-induced degranulation of human eosinophils in allergic asthmatic patients in vitro.

作者信息

Górski P, Palczyński C, Grzegorczyk J

机构信息

Department of Pneumonology and Allergology, Medical Academy of Lodz, Poland.

出版信息

Allergol Immunopathol (Madr). 1988 Jul-Aug;16(4):249-51.

PMID:2852468

Abstract

The aim of the study was to trace the response of eosinophil granulocyte to deuterium oxide in a group of atopic asthmatic patients. The study was performed in 20 asthmatic patients and 10 healthy subjects. Eosinophils were isolated on sodium metrizoate and then degranulated in deuterium oxide at the concentrations of 5, 25, 44, 50, 75 and 100%. Eosinophils were counted in a Fuchs-Rosenthal haemocythometric. In 7 of 20 patients eosinophil reaction did not differ from this in normal subjects. In 13 asthmatic patients the eosinophil degranulation in 44% D2O or more was stated. The results suggest that eosinophils are hyperresponsive to non-allergic stimuli in atopic asthmatics.

摘要

该研究的目的是追踪一组特应性哮喘患者中嗜酸性粒细胞对重水的反应。该研究在20名哮喘患者和10名健康受试者中进行。嗜酸性粒细胞在甲泛影酸钠上分离，然后在浓度为5%、25%、44%、50%、75%和100%的重水中脱颗粒。在Fuchs-Rosenthal血细胞计数板中对嗜酸性粒细胞进行计数。20名患者中有7名的嗜酸性粒细胞反应与正常受试者无异。在13名哮喘患者中，观察到在44%或更高浓度的重水中嗜酸性粒细胞脱颗粒。结果表明，特应性哮喘患者的嗜酸性粒细胞对非过敏性刺激反应过度。