Nazaktabar Ahmad, Lashkenari Mohammad Soleimani, Araghi Atefeh, Ghorbani Mohsen, Golshahi Hannaneh
Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran.
Faculty of Engineering Thechnologies, Amol University of Special Modern Technologies, Amol, Iran.
Int J Biol Macromol. 2017 Oct;103:379-384. doi: 10.1016/j.ijbiomac.2017.05.069. Epub 2017 May 17.
Evaluation of the potential cytotoxicity of polyrhodanine nanoparticles is an important factor for its biological applications. In current study, for the first time histopathological and biochemical analysis of polyrhodanine besides of its antiviral activity against Newcastle disease virus (NDV) were examined on chicken embryo model. Polyrhodanine was synthesized by the chemical oxidative polymerization method. The obtained nanoparticles were characterized by scanning electron microscopy (SEM), and Fourier transform infrared (FTIR). Different doses of polyrhodanine nanoparticles were injected into the albumen in 4-day-old embryonic eggs for groups: (0.1ppm, 1ppm, 10ppm and 100ppm), while the Control group received only normal saline. The gross examination of chicks revealed no abnormality. No pathological changes were detected in microscopical examination of the liver, kidney, spleen, heart, bursa of Fabricius and central nervous system tissues. Blood serum biochemical indices showed no significant differences between control and treatment groups. Interestingly, polyrhodanine nanoparticles showed strong antiviral activity against NDV in ovo. These preliminary findings suggest that polyrhodanine nanoparticles without any toxicity effect could be utilized in controlling Newcastle disease in chickens.
评估聚硫代罗丹宁纳米颗粒的潜在细胞毒性是其生物应用的一个重要因素。在当前研究中，首次在鸡胚模型上除了检测聚硫代罗丹宁对新城疫病毒（NDV）的抗病毒活性外，还对其进行了组织病理学和生化分析。聚硫代罗丹宁通过化学氧化聚合法合成。所获得的纳米颗粒通过扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）进行表征。将不同剂量的聚硫代罗丹宁纳米颗粒注射到4日龄胚胎蛋的蛋白中，分为以下几组：（0.1ppm、1ppm、10ppm和100ppm），而对照组仅接受生理盐水。雏鸡的大体检查未发现异常。在肝脏、肾脏、脾脏、心脏、法氏囊和中枢神经系统组织的显微镜检查中未检测到病理变化。血清生化指标显示对照组和治疗组之间无显著差异。有趣的是，聚硫代罗丹宁纳米颗粒在鸡胚内对NDV表现出强大的抗病毒活性。这些初步发现表明，无任何毒性作用的聚硫代罗丹宁纳米颗粒可用于控制鸡的新城疫。
