Donaldson K, Slight J, Bolton R E
Institute of Occupational Medicine, Roxburgh Place, Edinburgh.
Clin Exp Immunol. 1988 Dec;74(3):477-82.
Neutrophils and alveolar macrophages are found together in the alveolar region during pulmonary inflammation where neutrophil products could influence important macrophage defensive functions. We set out therefore to investigate the ability of neutrophil products to modulate alveolar macrophage phagocytosis and oxidant production. Neutrophils derived from acutely inflamed rat lung were incubated along with a range of potential triggers of neutrophil secretion and supernatants collected. Using two quantitative assays of rat alveolar macrophage phagocytosis, the supernatants were found to have no effect except for the quartz supernatant, which slightly enhanced phagocytosis via non-specific receptors and the PMA supernatant, which caused reduction in phagocytosis via non-specific and Fc receptors; this reduction could however be mimicked by PMA alone. None of the supernatants affected macrophage production of superoxide anion or hydrogen peroxide except for the PMA supernatant and once again the inhibitory effect of the PMA supernatant could be mimicked with PMA alone. It is concluded that products of inflammatory neutrophils do not affect phagocytosis or oxidative metabolism of alveolar macrophages, although in quartz-exposed lung neutrophils may exert a small enhancing effect on macrophage phagocytosis.
在肺部炎症期间，中性粒细胞和肺泡巨噬细胞共同存在于肺泡区域，中性粒细胞产生的物质可能会影响巨噬细胞重要的防御功能。因此，我们着手研究中性粒细胞产生的物质对肺泡巨噬细胞吞噬作用和氧化剂生成的调节能力。将源自急性炎症大鼠肺的中性粒细胞与一系列中性粒细胞分泌的潜在触发物一起孵育，并收集上清液。通过两种大鼠肺泡巨噬细胞吞噬作用的定量测定方法发现，除了石英上清液通过非特异性受体略微增强吞噬作用以及PMA上清液通过非特异性和Fc受体导致吞噬作用降低外，其他上清液均无影响；然而，单独使用PMA也可模拟这种降低作用。除了PMA上清液外，没有一种上清液影响巨噬细胞超氧阴离子或过氧化氢的生成，并且PMA上清液的抑制作用同样可以单独用PMA模拟。得出的结论是，炎症中性粒细胞产生的物质不会影响肺泡巨噬细胞的吞噬作用或氧化代谢，尽管在接触石英的肺中，中性粒细胞可能会对巨噬细胞吞噬作用产生轻微的增强作用。
