Gupta R K, Morton D L
J Surg Oncol. 1979;11(1):65-74. doi: 10.1002/jso.2930110112.
Tumor-associated antigens were demonstrated in concentrated and dialyzed urine of several sarcoma patients with large tumor burden. The antigens were detected by complement fixation using autologous and allogeneic sera from sarcoma patients. The antigenic activity in three patients who were studied sequentially disappeared after surgical ablation of tumor. In two of these three patients, the antigenic activity reappeared before tumor recurrence. The reactivity of the sarcoma sera to the urine could be abolished by absorption of the sera with human sarcoma cells but not by normal human liver cells, which indicates that the same antigen was present in the urine and on biopsy-obtained sarcoma cells. Urine from cancer patients with high tumor burden may be useful as a source of tumor-associated antigen. Further studies on the presence of these antigens in urine of sarcoma patients may lead to a method for detecting subclinical tumor recurrence.
在几名肿瘤负荷较大的肉瘤患者经浓缩和透析的尿液中证实了肿瘤相关抗原。使用肉瘤患者的自体血清和同种异体血清通过补体结合试验检测这些抗原。对三名患者进行了连续研究，肿瘤手术切除后，其抗原活性消失。在这三名患者中的两名患者中，抗原活性在肿瘤复发前再次出现。肉瘤血清与尿液的反应性可通过用人肉瘤细胞吸收血清而消除，但不能通过正常人肝细胞消除，这表明尿液和活检获得的肉瘤细胞中存在相同的抗原。肿瘤负荷高的癌症患者的尿液可能是肿瘤相关抗原的有用来源。对肉瘤患者尿液中这些抗原存在情况的进一步研究可能会导致一种检测亚临床肿瘤复发的方法。
