Tricard Jérémy, Ponsonnard Sébastien, Tholance Yannick, Mesturoux Laura, Lachatre Denis, Couquet Claude, Terro Faraj, Yardin Catherine, Marquet Pierre, Piccardo Alessandro, Gauthier Tristan
Department of Thoracic and Cardiovascular Surgery and Angiology, Limoges University Hospital Center, 87000 Limoges, France; Department of Pharmacology, Toxicology and Pharmacovigilance, INSERM UMR S-850, Limoges, France; Equipe de Recherche Médicale Appliquée (ERMA), University of Limoges, 87000, France.
Department of Anesthesia and Intensive Care, Limoges University Hospital Center, 87000 Limoges, France.
Eur J Obstet Gynecol Reprod Biol. 2017 Jul;214:162-167. doi: 10.1016/j.ejogrb.2017.05.013. Epub 2017 May 16.
To assess how the uterus tolerates extended cold ischemic storage before auto-transplantation in ewes.
Fourteen uterine auto-transplantations were performed in ewes from November 2014 to June 2015 at the Analysis and Research Laboratory of Limoges, France. The animals were divided into 2 groups: 7 after 3h of cold ischemia timeand 7 after 24h. Transplant was assessed ≥8days after transplantation. Histology and apoptosis analyses (TUNEL method and indirect immunohistochemistry of cleaved Caspase 3) were performed before uterus retrieval (control), after 90min following reperfusion and ≥8days after transplantation.
Twelve uterine auto-transplantations were successfully performed. The histological analysis at 90min following reperfusion revealed a moderate inflammation of the endometrium and serosa in the 3-h group and severe inflammation in the 24-h group, but no significant apoptotic signal was found in either group. Seven ewes were alive at ≥8days after transplantation: the macroscopic and histological analyses revealed two viable uteri in the 3-h group and three in the 24-h group. In each group one uterus was necrotic.
These first results in ewes suggest that the uterus is an organ with a good tolerance to extended cold ischemic storage before transplantation.
评估在绵羊自体移植前子宫对延长冷缺血储存的耐受情况。
2014年11月至2015年6月在法国利摩日分析与研究实验室对绵羊进行了14例子宫自体移植。动物被分为两组：7例冷缺血时间为3小时，7例为24小时。移植后≥8天进行评估。在子宫取出前（对照）、再灌注90分钟后以及移植后≥8天进行组织学和凋亡分析（TUNEL法和裂解的半胱天冬酶3间接免疫组化）。
成功进行了12例子宫自体移植。再灌注90分钟时的组织学分析显示，3小时组子宫内膜和浆膜有中度炎症，24小时组有重度炎症，但两组均未发现明显的凋亡信号。7只母羊在移植后≥8天存活：大体和组织学分析显示，3小时组有2个存活子宫，24小时组有3个。每组有1个子宫坏死。
绵羊的这些初步结果表明，子宫是一个在移植前对延长冷缺血储存具有良好耐受性的器官。
