Stojanović T, Mrsulja B B
Institute of Biochemistry, School of Medicine, University of Belgrade, Yugoslavia.
Metab Brain Dis. 1988 Dec;3(4):265-72. doi: 10.1007/BF00999536.
The present experiments were designed to determine the kinetic pattern of Na,K-ATPase in the presence of varying concentrations of Na+ and K+ ions in controls and gerbils exposed to 1 and 5 min of ischemia, respectively, and 60 min and 4 days of recirculation following 5 min of transient ischemia. The pattern of Na,K-ATPase activity in the control cerebral cortex and hippocampus is different. The cortical Na,K-ATPase apparently is more resistant in keeping an optimal activity than the enzyme in the hippocampus. After ischemic insult of either 1 or 5 min in duration, the enzyme activity is inhibited in both brain structures. 4 days after 5 min of ischemia, indicating greater flexibility of the cortical enzyme or less damage than in the hippocampus. Furthermore, the data obtained show that only through the enzyme behavior and kinetic parameters is it possible to reach conclusions about the enzyme function or dysfunction under pathological conditions.
本实验旨在分别测定在对照动物以及经历1分钟和5分钟缺血的沙鼠中，在不同浓度的Na⁺和K⁺离子存在下，Na,K-ATP酶的动力学模式，以及在5分钟短暂缺血后再灌注60分钟和4天的情况下该酶的动力学模式。对照大脑皮层和海马体中Na,K-ATP酶的活性模式不同。皮层中的Na,K-ATP酶在维持最佳活性方面显然比海马体中的该酶更具抗性。在持续1分钟或5分钟的缺血性损伤后，这两个脑区结构中的酶活性均受到抑制。缺血5分钟后4天，表明皮层酶具有更大的灵活性或比海马体中的损伤更小。此外，所获得的数据表明，只有通过酶的行为和动力学参数，才有可能得出关于病理条件下酶功能或功能障碍的结论。
