State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
College of Hydraulic and Hydroelectric Engineering, Sichuan University, Chengdu 610041, China.
Arch Oral Biol. 2017 Sep;81:151-159. doi: 10.1016/j.archoralbio.2017.05.005. Epub 2017 May 17.
The objective of this study was to investigate the specific roles of follicular dendritic cell secreted protein (FDC-SP), a protein exists in saliva, in the inhibition of calcium precipitation during periodontal regeneration, as well as affect phenotype expression of human periodontal ligament cells (hPDLCs) during the differentiation process.
To investigate this, we applied microarray technology to identify gene expression changes in hPDLCs transfected with FDC-SP and then clustered them according to their biological functions.
One hundred seventy-one genes were found differentially expressed by at least two-fold between FDC-SP -transfected and empty vector-transfected cells. Besides, genes encoding cell-cycle proteins, blood-related and cell differentiation-related proteins tended to be up-regulated after FDC-SP transfection, whereas cytokine/growth factors, signal transduction and metabolism-related genes tended to be down-regulated in hPDLCs overexpression FDC-SP.
The present study investigated FDC-SP's roles in hPDLCs' phenotype expression, via comparing the gene expression profiles between FDC-SP -transfected hPDLCs and empty vector-transfected cells upon microarray analysis. hPDLCs overexpression FDC-SP appear to display different gene expression patterns. In all, these observations showed a potential of FDC-SP in the maintenance of PDL homeostasis and its ultimate contribution to periodontal would-healing processes.
本研究旨在探讨存在于唾液中的滤泡树突状细胞分泌蛋白（FDC-SP）在牙周再生过程中抑制钙沉淀的具体作用，以及其在人牙周膜细胞（hPDLC）分化过程中对细胞表型表达的影响。
为了研究这一点，我们应用微阵列技术来鉴定转染 FDC-SP 的 hPDLC 中的基因表达变化，并根据其生物学功能对其进行聚类。
在转染 FDC-SP 的细胞和空载体转染的细胞之间，发现有 171 个基因的表达差异至少有两倍。此外，细胞周期蛋白、血液相关和细胞分化相关蛋白的基因在转染 FDC-SP 后倾向于上调，而细胞因子/生长因子、信号转导和代谢相关基因在 hPDLC 中过表达 FDC-SP 时则倾向于下调。
本研究通过微阵列分析比较了转染 FDC-SP 的 hPDLC 和空载体转染细胞之间的基因表达谱，研究了 FDC-SP 对 hPDLC 细胞表型表达的作用。过表达 FDC-SP 的 hPDLC 似乎表现出不同的基因表达模式。总之，这些观察结果表明 FDC-SP 具有维持牙周组织内稳态的潜力，并最终有助于牙周组织的愈合过程。
