Shirahama M, Ishibashi H, Tsuchiya Y, Kurokawa S, Okumura Y, Niho Y
First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
J Clin Lab Immunol. 1988 Nov;27(3):127-32.
Rat Kupffer cells stimulated with bacterial lipopolysaccharide (LPS) produced high levels of interleukin 1 (IL-1), determined by assaying the thymocyte proliferating activity. The unstimulated Kupffer cells secreted no detectable activity. LPS-induced production of IL-1 activity was dose-dependent and as little as 0.1 micrograms/ml of LPS induced the IL-1 production. Thirty minutes were required for LPS to induce sufficient amounts of IL-1 activity. IL-2 activity was not detected in the Kupffer cell culture medium. Another soluble agent, N-acetylmuramyl-L-alanyl-D-isoglutamine, or phagocytable agents, silica and zymosan, also stimulated IL-1 production by Kupffer cells, whereas phorbol myristate acetate did not. Kinetic studies revealed a rapid increase in both intra- and extracellular IL-1 activity within 1 hr following the addition of LPS, with a peak at 6 hr. This IL-1 activity produced by Kupffer cells was heat labile, resistant to freezing and thawing and precipitated with 65% ammonium sulfate. Upon gel filtration, the activity was present in three peaks of approximately 110, 35 and 15 kd. The hepatocyte stimulating activity, when assayed by alpha 2-macroglobulin inducing activity, was detected in the first two peaks but not in the third peak. These studies suggest that LAF activity is distinct from hepatocyte stimulating activity at least in the molecular weight.
通过检测胸腺细胞增殖活性发现，用细菌脂多糖（LPS）刺激大鼠枯否细胞可产生高水平的白细胞介素1（IL-1）。未受刺激的枯否细胞不分泌可检测到的活性物质。LPS诱导的IL-1活性产生呈剂量依赖性，低至0.1微克/毫升的LPS即可诱导IL-1的产生。LPS诱导产生足够量的IL-1活性需要30分钟。在枯否细胞培养基中未检测到IL-2活性。另一种可溶性物质N-乙酰胞壁酰-L-丙氨酰-D-异谷氨酰胺，或可吞噬物质二氧化硅和酵母聚糖，也可刺激枯否细胞产生IL-1，而佛波酯肉豆蔻酸酯则不能。动力学研究显示，添加LPS后1小时内细胞内和细胞外的IL-1活性均迅速增加，6小时时达到峰值。枯否细胞产生的这种IL-1活性对热不稳定，耐冻融，可被65%硫酸铵沉淀。经凝胶过滤后，活性存在于三个峰中，分子量分别约为110、35和15千道尔顿。通过α2-巨球蛋白诱导活性检测肝细胞刺激活性时，在前两个峰中检测到了该活性，而在第三个峰中未检测到。这些研究表明，至少在分子量方面，白细胞激活因子活性与肝细胞刺激活性不同。
