State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China.
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China.
Plant Sci. 2017 Aug;261:18-27. doi: 10.1016/j.plantsci.2017.05.002. Epub 2017 May 15.
RNA binding proteins play important roles in plant responses to biotic and abiotic stresses. The YT521-B homology (YTH) domain-containing RNA binding protein (YTP) was first found in Rattus norvegicus and is related to oxygen-deficient stress. The Malus YTP gene family has 15 members. Results from their functional analysis will help researchers improve stress tolerance and fruit quality in apple. We cloned two homologous YTP family members in M. hupehensis - MhYTP1 and MhYTP2 - and identified their promoter regions that contain many cis-elements related to biotic and abiotic stresses. Both MhYTP1 and MhYTP2 can be induced by various treatments, e.g., methyl jasmonate (MeJA), salicylic acid (SA), abscisic acid (ABA), water-logging, water deficits, and high salinity. When compared with the wild type (WT), transgenic plants of 'GL-3' ('Royal Gala') apple that over-express MhYTP1 or MhYTP2 are more sensitive to D. mali infection, heat stress, and high salinity, more resistant to water-logging, chilling, drought and nutrition deficient conditions. All of these findings indicate that MhYTP1 and MhYTP2 participate in various biotic- and abiotic-stress responses.
RNA 结合蛋白在植物应对生物和非生物胁迫中发挥着重要作用。YT521-B 同源（YTH）结构域含有 RNA 结合蛋白（YTP）最初在挪威鼠中发现，与缺氧胁迫有关。苹果的 YTP 基因家族有 15 个成员。对其功能进行分析的结果将有助于研究人员提高苹果的抗逆性和果实品质。我们在 M. hupehensis 中克隆了两个同源的 YTP 家族成员 - MhYTP1 和 MhYTP2 - 并鉴定了它们的启动子区域，其中包含许多与生物和非生物胁迫相关的顺式元件。MhYTP1 和 MhYTP2 均可被多种处理诱导，如茉莉酸甲酯（MeJA）、水杨酸（SA）、脱落酸（ABA）、淹水、水分亏缺和高盐。与野生型（WT）相比，过表达 MhYTP1 或 MhYTP2 的‘GL-3’（‘皇家 Gala’）苹果转基因植株对 D. mali 感染、热胁迫和高盐更为敏感，对淹水、冷胁迫、干旱和营养缺乏条件更为耐受。所有这些发现表明，MhYTP1 和 MhYTP2 参与了各种生物和非生物胁迫反应。
